Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EJN60_RS10240 Genome accession   NZ_LR130533
Coordinates   2075422..2077122 (+) Length   566 a.a.
NCBI ID   WP_088355580.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa isolate paerg009     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2070422..2082122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN60_RS10215 (BANRA_01982) ampD 2070433..2070999 (-) 567 WP_023096121.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EJN60_RS10220 (BANRA_01983) - 2071150..2073405 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  EJN60_RS10225 (BANRA_01984) nadC 2073691..2074539 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EJN60_RS10235 pilA 2074738..2075190 (-) 453 WP_031636800.1 pilin Machinery gene
  EJN60_RS10240 (BANRA_01986) pilB 2075422..2077122 (+) 1701 WP_088355580.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EJN60_RS10245 (BANRA_01987) pilC 2077126..2078346 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  EJN60_RS10250 (BANRA_01988) pilD 2078350..2079222 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EJN60_RS10255 (BANRA_01989) coaE 2079219..2079830 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  EJN60_RS10260 (BANRA_01990) yacG 2079827..2080027 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  EJN60_RS10265 (BANRA_01991) - 2080064..2080273 (-) 210 WP_003094660.1 hypothetical protein -
  EJN60_RS10270 (BANRA_01992) - 2080379..2081068 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  EJN60_RS10275 (BANRA_01993) - 2081065..2081535 (-) 471 WP_023096122.1 hypothetical protein -
  EJN60_RS10280 (BANRA_01994) - 2081532..2081957 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62355.26 Da        Isoelectric Point: 5.8580

>NTDB_id=1117128 EJN60_RS10240 WP_088355580.1 2075422..2077122(+) (pilB) [Pseudomonas aeruginosa isolate paerg009]
MNDSIQLSGLSRQLVQANLLDEKTALQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
TISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQAPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1117128 EJN60_RS10240 WP_088355580.1 2075422..2077122(+) (pilB) [Pseudomonas aeruginosa isolate paerg009]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCTCTGCA
GGCGCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGTATCGCCTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
ACCATCAGCGAAAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTAGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG
GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCTGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGCCCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGGCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGACGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.343

100

0.58
  pilB Acinetobacter baylyi ADP1

57.016

99.47

0.567
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.549
  pilB Vibrio parahaemolyticus RIMD 2210633

50.709

99.647

0.505
  pilF Neisseria gonorrhoeae MS11

50.356

99.293

0.5
  pilB Vibrio cholerae strain A1552

49.823

99.647

0.496
  pilB Vibrio campbellii strain DS40M4

49.468

99.647

0.493
  pilF Thermus thermophilus HB27

39.891

96.996

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

92.756

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

38.764

94.346

0.366

Multiple sequence alignment