Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   AT689_RS03685 Genome accession   NZ_LN831051
Coordinates   701239..702180 (+) Length   313 a.a.
NCBI ID   WP_000249571.1    Uniprot ID   A0A0B7M3Q7
Organism   Streptococcus pneumoniae strain NCTC7465     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 696239..707180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT689_RS03665 (ERS445053_00717) - 696305..698122 (+) 1818 WP_001220841.1 acyltransferase family protein -
  AT689_RS03670 (ERS445053_00718) nagA 698275..699426 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  AT689_RS03675 (ERS445053_00719) - 699589..700647 (+) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  AT689_RS03680 (ERS445053_00720) - 700798..701163 (+) 366 WP_000286415.1 DUF1033 family protein -
  AT689_RS03685 (ERS445053_00721) comGA/cglA/cilD 701239..702180 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  AT689_RS03690 (ERS445053_00722) comGB/cglB 702128..703144 (+) 1017 WP_001844679.1 competence type IV pilus assembly protein ComGB Machinery gene
  AT689_RS03695 (ERS445053_00723) comGC/cglC 703146..703472 (+) 327 WP_000738633.1 comG operon protein ComGC Machinery gene
  AT689_RS03700 (ERS445053_00724) comGD/cglD 703465..703869 (+) 405 WP_000588020.1 competence type IV pilus minor pilin ComGD Machinery gene
  AT689_RS03705 (ERS445053_00725) comGE/cglE 703832..704134 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  AT689_RS03710 (ERS445053_00726) comGF/cglF 704097..704558 (+) 462 WP_000250531.1 competence type IV pilus minor pilin ComGF Machinery gene
  AT689_RS03715 (ERS445053_00727) comGG/cglG 704536..704949 (+) 414 WP_000265631.1 competence type IV pilus minor pilin ComGG Machinery gene
  AT689_RS12440 - 705086..705680 (+) 595 Protein_717 class I SAM-dependent methyltransferase -
  AT689_RS03730 (ERS445053_00729) comYH 705741..706694 (+) 954 WP_000345130.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35591.51 Da        Isoelectric Point: 6.0083

>NTDB_id=1114365 AT689_RS03685 WP_000249571.1 701239..702180(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain NCTC7465]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1114365 AT689_RS03685 WP_000249571.1 701239..702180(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain NCTC7465]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGACATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M3Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361

Multiple sequence alignment