Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   AT689_RS03690 Genome accession   NZ_LN831051
Coordinates   702128..703144 (+) Length   338 a.a.
NCBI ID   WP_001844679.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain NCTC7465     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697128..708144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT689_RS03670 (ERS445053_00718) nagA 698275..699426 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  AT689_RS03675 (ERS445053_00719) - 699589..700647 (+) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  AT689_RS03680 (ERS445053_00720) - 700798..701163 (+) 366 WP_000286415.1 DUF1033 family protein -
  AT689_RS03685 (ERS445053_00721) comGA/cglA/cilD 701239..702180 (+) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  AT689_RS03690 (ERS445053_00722) comGB/cglB 702128..703144 (+) 1017 WP_001844679.1 competence type IV pilus assembly protein ComGB Machinery gene
  AT689_RS03695 (ERS445053_00723) comGC/cglC 703146..703472 (+) 327 WP_000738633.1 comG operon protein ComGC Machinery gene
  AT689_RS03700 (ERS445053_00724) comGD/cglD 703465..703869 (+) 405 WP_000588020.1 competence type IV pilus minor pilin ComGD Machinery gene
  AT689_RS03705 (ERS445053_00725) comGE/cglE 703832..704134 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  AT689_RS03710 (ERS445053_00726) comGF/cglF 704097..704558 (+) 462 WP_000250531.1 competence type IV pilus minor pilin ComGF Machinery gene
  AT689_RS03715 (ERS445053_00727) comGG/cglG 704536..704949 (+) 414 WP_000265631.1 competence type IV pilus minor pilin ComGG Machinery gene
  AT689_RS12440 - 705086..705680 (+) 595 Protein_717 class I SAM-dependent methyltransferase -
  AT689_RS03730 (ERS445053_00729) comYH 705741..706694 (+) 954 WP_000345130.1 class I SAM-dependent methyltransferase Machinery gene
  AT689_RS03735 (ERS445053_00730) - 706745..707935 (+) 1191 WP_000167766.1 acetate kinase -
  AT689_RS13375 - 707936..708067 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38535.63 Da        Isoelectric Point: 9.6162

>NTDB_id=1114366 AT689_RS03690 WP_001844679.1 702128..703144(+) (comGB/cglB) [Streptococcus pneumoniae strain NCTC7465]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMAGLVSVLALLALTFYKRSSKMNVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1114366 AT689_RS03690 WP_001844679.1 702128..703144(+) (comGB/cglB) [Streptococcus pneumoniae strain NCTC7465]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGTTTTTTCTTCTCTTAATCATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGCAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAATGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.379

100

0.944
  comGB/cglB Streptococcus mitis NCTC 12261

93.787

100

0.938
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.044

93.787

0.544
  comYB Streptococcus mutans UA159

58.044

93.787

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment