Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GJA_RS21080 Genome accession   NZ_HG322949
Coordinates   4905991..4907127 (+) Length   378 a.a.
NCBI ID   WP_038496197.1    Uniprot ID   W0VCX5
Organism   Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4900991..4912127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJA_RS21060 (GJA_4541) - 4901595..4903112 (+) 1518 WP_038496189.1 PLP-dependent aminotransferase family protein -
  GJA_RS21065 (GJA_4542) proC 4903189..4904037 (-) 849 WP_038496191.1 pyrroline-5-carboxylate reductase -
  GJA_RS21070 (GJA_4543) - 4904087..4904788 (-) 702 WP_038496193.1 YggS family pyridoxal phosphate-dependent enzyme -
  GJA_RS21075 (GJA_4544) pilT 4904905..4905948 (+) 1044 WP_038496195.1 type IV pilus twitching motility protein PilT Machinery gene
  GJA_RS21080 (GJA_4545) pilU 4905991..4907127 (+) 1137 WP_038496197.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GJA_RS21085 (GJA_4546) - 4907127..4907618 (+) 492 WP_038496199.1 glutathione peroxidase -
  GJA_RS21090 (GJA_4548) - 4907828..4909909 (+) 2082 WP_038496202.1 GGDEF domain-containing protein -
  GJA_RS21095 (GJA_4547) - 4909906..4910958 (-) 1053 WP_144241613.1 hypothetical protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41860.29 Da        Isoelectric Point: 6.4312

>NTDB_id=1111908 GJA_RS21080 WP_038496197.1 4905991..4907127(+) (pilU) [Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628]
MERDQASKLMSDLLRLMVSKNGSDLFITAGFPPAIKIDGKLTPVSPQVLSAAHTVDLARSIMNDKQIASFELSKEANFAL
SPPDLGRFRVSAFVQMGCVGMVLRTINTAIPKLEDLGLPTVLQDVVMSKRGLVIMVGATGSGKSTTLAAMVGYRNENSYG
HIITIEDPVEYVHPHKNCIVTQREVGVDTDDWEIALKNTLRQAPDVIQIGEIRDRETMDHAISFAETGHLCLATLHANSA
NQALDRIVNFFPEERRQQLLMDLSLNLRGMISQRLVPKKETKGRCVAMEIMLNSPLISDLIFKGNVHEIKEIMKKSRELG
MQTFDQALFDLFEADQISYEDALRNADSVNDLRLSIKLHGKAAHTRDLSAGTEHLGLI

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1111908 GJA_RS21080 WP_038496197.1 4905991..4907127(+) (pilU) [Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628]
ATGGAACGGGACCAGGCTTCAAAACTCATGTCGGACTTGCTGCGGCTGATGGTCAGCAAGAACGGCTCCGACCTGTTCAT
CACCGCAGGCTTTCCGCCGGCCATCAAGATCGACGGCAAGCTGACGCCGGTGTCGCCGCAAGTCCTGAGCGCGGCCCACA
CGGTCGACCTGGCGCGCTCGATCATGAACGACAAGCAAATCGCGTCGTTCGAACTGAGCAAGGAAGCCAATTTCGCGCTC
AGCCCGCCAGACCTGGGCCGCTTCCGCGTGTCGGCCTTCGTGCAGATGGGTTGTGTCGGCATGGTCTTGCGCACGATCAA
TACCGCGATCCCGAAACTGGAAGACCTCGGCTTGCCGACCGTGCTGCAAGACGTGGTGATGAGCAAGCGCGGCCTGGTGA
TCATGGTCGGCGCCACCGGTTCCGGCAAATCGACGACGCTGGCGGCGATGGTCGGCTACCGCAATGAAAACAGTTACGGC
CACATCATCACCATCGAAGATCCGGTCGAATACGTGCATCCGCACAAAAATTGCATCGTCACGCAACGTGAAGTAGGCGT
CGATACCGACGATTGGGAAATCGCGTTGAAAAACACGCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAAATCCGCG
ACCGCGAAACGATGGACCACGCCATCTCGTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGTATCGTCAACTTTTTCCCCGAAGAGCGGCGCCAGCAATTGCTGATGGATTTGTCGCTGAACTT
GCGCGGCATGATCTCGCAGCGCCTGGTGCCGAAGAAGGAAACCAAGGGGCGCTGCGTGGCGATGGAAATCATGTTAAATT
CACCATTGATATCGGACTTGATCTTCAAGGGCAATGTGCATGAAATCAAGGAAATCATGAAAAAGTCGCGCGAACTGGGC
ATGCAAACGTTCGACCAGGCGCTGTTCGACTTGTTCGAAGCCGATCAAATCAGTTACGAAGATGCCTTGCGCAACGCCGA
TTCCGTCAACGACTTGCGCCTGTCCATCAAATTGCATGGCAAGGCAGCGCACACCCGCGATCTGTCTGCCGGAACCGAAC
ATTTAGGATTAATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0VCX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.295

91.534

0.579
  pilU Acinetobacter baylyi ADP1

57.627

93.651

0.54
  pilU Vibrio cholerae strain A1552

57.771

90.212

0.521
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

89.153

0.392
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.136

89.153

0.376
  pilT Acinetobacter baumannii strain A118

42.342

88.095

0.373
  pilT Acinetobacter baumannii D1279779

42.342

88.095

0.373
  pilT Acinetobacter nosocomialis M2

42.342

88.095

0.373
  pilT Acinetobacter baylyi ADP1

43.034

85.45

0.368
  pilT Legionella pneumophila strain Lp02

41.441

88.095

0.365
  pilT Legionella pneumophila strain ERS1305867

41.441

88.095

0.365

Multiple sequence alignment