Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HV97_RS26490 Genome accession   NZ_CP008870
Coordinates   5542920..5543372 (-) Length   150 a.a.
NCBI ID   WP_024947914.1    Uniprot ID   A4GRK7
Organism   Pseudomonas aeruginosa strain W36662     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5537920..5548372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV97_RS26470 (HV97_22820) ampD 5538626..5539192 (-) 567 WP_023084738.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HV97_RS26475 (HV97_22825) - 5539343..5541598 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  HV97_RS26480 (HV97_22830) nadC 5541884..5542732 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HV97_RS26490 (HV97_22840) pilA 5542920..5543372 (-) 453 WP_024947914.1 pilin Machinery gene
  HV97_RS26495 (HV97_22845) pilB 5543601..5545301 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HV97_RS26500 (HV97_22850) - 5545305..5545601 (+) 297 Protein_5130 type II secretion system F family protein -
  HV97_RS26505 (HV97_22855) - 5545842..5547113 (-) 1272 WP_004423958.1 IS4-like element ISPa1635 family transposase -
  HV97_RS26515 (HV97_33070) - 5547238..5548170 (+) 933 Protein_5132 type II secretion system F family protein -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15622.02 Da        Isoelectric Point: 8.4827

>NTDB_id=110370 HV97_RS26490 WP_024947914.1 5542920..5543372(-) (pilA) [Pseudomonas aeruginosa strain W36662]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALASVNPLKTTVEEALSRGWSVKSGTGTEDATKKEVPLG
VAADANKLGTIALKPDPADGTADITLTFTMGGAGPKNKGKIITLTRTAADGLWKCTSDQDEQFIPKGCSK

Nucleotide


Download         Length: 453 bp        

>NTDB_id=110370 HV97_RS26490 WP_024947914.1 5542920..5543372(-) (pilA) [Pseudomonas aeruginosa strain W36662]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCTTGGCTGCAATTGCCAT
TCCTCAGTATCAGAATTATGTAGCTCGTTCGGAAGGCGCATCTGCTCTTGCTTCGGTCAATCCGTTGAAGACTACCGTTG
AAGAGGCGCTTTCTCGTGGTTGGAGCGTGAAGAGCGGTACAGGTACAGAGGACGCTACTAAGAAAGAGGTTCCTCTGGGG
GTGGCGGCAGATGCTAACAAACTGGGTACTATCGCACTCAAACCCGATCCTGCTGATGGTACTGCAGATATTACTTTGAC
TTTCACTATGGGCGGTGCAGGACCGAAGAATAAAGGGAAAATTATTACCCTGACTCGTACTGCAGCTGATGGTCTCTGGA
AGTGCACCAGTGATCAGGATGAGCAGTTTATTCCGAAAGGTTGCTCTAAGTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A4GRK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

100

100

1
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.682

100

0.48
  pilA Acinetobacter baumannii strain A118

45.333

100

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.575

97.333

0.453
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.675

100

0.447
  pilA Vibrio cholerae C6706

42.675

100

0.447
  pilA Vibrio cholerae strain A1552

42.675

100

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

48.507

89.333

0.433
  comP Acinetobacter baylyi ADP1

40.789

100

0.413
  pilA2 Legionella pneumophila strain ERS1305867

39.865

98.667

0.393
  pilA/pilA1 Eikenella corrodens VA1

39.456

98

0.387
  pilA2 Legionella pneumophila str. Paris

39.189

98.667

0.387

Multiple sequence alignment