Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HV97_RS26495 Genome accession   NZ_CP008870
Coordinates   5543601..5545301 (+) Length   566 a.a.
NCBI ID   WP_009875880.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain W36662     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5538601..5550301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HV97_RS26470 (HV97_22820) ampD 5538626..5539192 (-) 567 WP_023084738.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HV97_RS26475 (HV97_22825) - 5539343..5541598 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  HV97_RS26480 (HV97_22830) nadC 5541884..5542732 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HV97_RS26490 (HV97_22840) pilA 5542920..5543372 (-) 453 WP_024947914.1 pilin Machinery gene
  HV97_RS26495 (HV97_22845) pilB 5543601..5545301 (+) 1701 WP_009875880.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HV97_RS26500 (HV97_22850) - 5545305..5545601 (+) 297 Protein_5130 type II secretion system F family protein -
  HV97_RS26505 (HV97_22855) - 5545842..5547113 (-) 1272 WP_004423958.1 IS4-like element ISPa1635 family transposase -
  HV97_RS26515 (HV97_33070) - 5547238..5548170 (+) 933 Protein_5132 type II secretion system F family protein -
  HV97_RS26520 (HV97_22870) pilD 5548174..5549046 (+) 873 WP_023087804.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HV97_RS26525 (HV97_22875) coaE 5549043..5549654 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  HV97_RS26530 (HV97_22880) yacG 5549651..5549851 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HV97_RS26535 (HV97_22885) - 5549888..5550097 (-) 210 WP_003094660.1 hypothetical protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62383.27 Da        Isoelectric Point: 5.5590

>NTDB_id=110371 HV97_RS26495 WP_009875880.1 5543601..5545301(+) (pilB) [Pseudomonas aeruginosa strain W36662]
MNDSIQLSGLSRQLVQANLLDEKTALQAQTQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDRESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDAANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFENATDGLAGLDDVDLE
GLDVGVKETSGQEDTGAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=110371 HV97_RS26495 WP_009875880.1 5543601..5545301(+) (pilB) [Pseudomonas aeruginosa strain W36662]
ATGAACGACAGCATCCAACTGAGCGGTCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCCTGCA
GGCCCAGACGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGACAGGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATTCCCCTGTGGCGACGGGGCAACAAGTTGTTCGTAGGCATCTC
CGACGCCGCCAACCACCAGGCCATCAACGACGTCCAGTTCAGCACTGGCCTGACGACCGAGGCCATTCTGGTCGAGGACG
ACAAGCTCGGCCTTGCCATCGACAAGCTATTCGAGAATGCAACCGACGGCTTGGCAGGTCTCGATGACGTGGACCTGGAA
GGACTGGATGTAGGCGTCAAGGAAACGTCCGGACAAGAAGATACTGGCGCGGAGGCAGACGACGCTCCCGTCGTCCGCTT
CGTCAACAAGATGCTGCTGGACGCTATCAAAGGCGGGTCTTCCGACTTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTTCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTGGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTACCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAGCACGACGTACCGAAGGAAACCCTGCTTCACGAAGGTTTCCCAGAA
GAGTTGATCGGCACCTTCAAGCTGTATTCACCAGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.719

100

0.581
  pilB Acinetobacter baylyi ADP1

57.371

99.47

0.571
  pilB Legionella pneumophila strain ERS1305867

54.77

100

0.548
  pilF Neisseria gonorrhoeae MS11

50.534

99.293

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.444

99.47

0.502
  pilB Vibrio cholerae strain A1552

50

100

0.5
  pilB Vibrio campbellii strain DS40M4

49.113

99.647

0.489
  pilF Thermus thermophilus HB27

39.855

97.527

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.417

93.11

0.376

Multiple sequence alignment