Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACM0CX_RS09425 Genome accession   NZ_CP180564
Coordinates   2049959..2051098 (-) Length   379 a.a.
NCBI ID   WP_095592453.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BJ018     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2044959..2056098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACM0CX_RS09405 feoB 2045177..2047450 (+) 2274 WP_411637017.1 Fe(2+) transporter permease subunit FeoB -
  ACM0CX_RS09410 - 2047447..2047689 (+) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  ACM0CX_RS09415 - 2047789..2048268 (-) 480 WP_045789457.1 WbuC family cupin fold metalloprotein -
  ACM0CX_RS09420 glgC 2048404..2049675 (-) 1272 WP_011706551.1 glucose-1-phosphate adenylyltransferase -
  ACM0CX_RS09425 pilU 2049959..2051098 (-) 1140 WP_095592453.1 type IVa pilus ATPase TapW Machinery gene
  ACM0CX_RS09430 - 2051159..2051515 (-) 357 WP_005302630.1 DMT family protein -
  ACM0CX_RS09435 - 2051629..2052360 (+) 732 WP_029300033.1 substrate-binding periplasmic protein -
  ACM0CX_RS09440 btuC 2052461..2053468 (+) 1008 WP_411637018.1 vitamin B12 ABC transporter permease BtuC -
  ACM0CX_RS09445 - 2053455..2054240 (+) 786 WP_065476865.1 ATP-binding cassette domain-containing protein -
  ACM0CX_RS09450 - 2054418..2055482 (-) 1065 WP_146061246.1 3-deoxy-7-phosphoheptulonate synthase -
  ACM0CX_RS09455 - 2055638..2056018 (+) 381 WP_005302618.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42305.37 Da        Isoelectric Point: 6.4290

>NTDB_id=1096641 ACM0CX_RS09425 WP_095592453.1 2049959..2051098(-) (pilU) [Aeromonas hydrophila strain BJ018]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1096641 ACM0CX_RS09425 WP_095592453.1 2049959..2051098(-) (pilU) [Aeromonas hydrophila strain BJ018]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTGCGTCCGCTCAATGAAACGCCGCTCGAGCCCGGCGAGGTGGCCAGAATAGCTGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAGTCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACTCTGCGTCAGGCACCCGACGTCATCCTCATCGGTGAAATTCGCGACCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCCGAGACCGGCCACCTGGCGATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAACTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
GCTGGTTAAGACTACTGATGGCGGTCGCCGGGCGGCAGTGGAGATCATGCTGGGCACCCACACCATTCGCGACATGATCA
AACGTGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTAGAAGGTGTGATCGACGAGGAGGAGGCAGTAAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGACG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

98.945

0.538
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.988

90.765

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii strain A118

41.317

88.127

0.364
  pilT Acinetobacter baumannii D1279779

41.317

88.127

0.364
  pilT Acinetobacter nosocomialis M2

41.317

88.127

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364

Multiple sequence alignment