Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACKWD1_RS08795 Genome accession   NZ_CP177201
Coordinates   1932135..1933271 (+) Length   378 a.a.
NCBI ID   WP_029314740.1    Uniprot ID   -
Organism   Aeromonas caviae strain QZ63     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 1929870..1931558 1932135..1933271 flank 577


Gene organization within MGE regions


Location: 1929870..1933271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWD1_RS08780 (ACKWD1_08780) tnpA 1929870..1930274 (-) 405 WP_010674034.1 IS200/IS605-like element ISAs26 family transposase -
  ACKWD1_RS08785 (ACKWD1_08785) - 1930332..1931558 (+) 1227 WP_041206018.1 RNA-guided endonuclease InsQ/TnpB family protein -
  ACKWD1_RS08790 (ACKWD1_08790) - 1931722..1932078 (+) 357 WP_201985102.1 DMT family protein -
  ACKWD1_RS08795 (ACKWD1_08795) pilU 1932135..1933271 (+) 1137 WP_029314740.1 type IVa pilus ATPase TapW Machinery gene

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42203.27 Da        Isoelectric Point: 6.6000

>NTDB_id=1086128 ACKWD1_RS08795 WP_029314740.1 1932135..1933271(+) (pilU) [Aeromonas caviae strain QZ63]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLTQEPLGPGEVARIAYDIMDLEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHQKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTVRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDEGDLF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1086128 ACKWD1_RS08795 WP_029314740.1 1932135..1933271(+) (pilU) [Aeromonas caviae strain QZ63]
ATGGAATTGAGAGACATGCTGCAGATCCTGGCCAAACAGGACGGTTCCGATCTCTATCTCTCCACCGGCGCCCCCCCCTG
CGCCAAGTTCAACGGCGCCCTGCGCCCCCTCACCCAGGAGCCCCTGGGGCCGGGCGAGGTGGCCCGCATCGCCTACGACA
TCATGGATCTCGAGCAGAAACAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAGCAGCGCAATGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTCAAACTCCCTCCGGTGCTCCTCGACACCATCATGGAGAAGCGGGGTCTGGTGCTCTTCGTCGGCGGCACGGGGT
CCGGCAAATCCACGTCGCTCGCGGCCCTCATCGATCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCAGAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATACTCATCGGCGAAATCCGCGATCGGGAGACCATGGAGCATG
CTCTCGCCTTCTCGGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCAGACCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
GCTGGTGAAGACCACGGACGGCGGGCGCCGGGCGGCGGTGGAGGTGATGCTCGGGACCCACACTGTGCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAAAAATCCAGGGCCCTTGGCATGGTGACCTTCGACAGCACTCTG
TTCGATCTGGTGGTCGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCGGACTCCCAGAACAACCTGCGCCTCAA
GATCAAACTCTGGCGGGAGAAGGGCCAGATTGGCAACAGCGCGGCGGCGAGCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGGGCGACCTCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.4

99.206

0.54
  pilU Acinetobacter baylyi ADP1

52.514

94.709

0.497
  pilU Vibrio cholerae strain A1552

49.858

93.386

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.478

89.683

0.381
  pilT Vibrio cholerae strain A1552

40.456

92.857

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

92.857

0.376
  pilT Legionella pneumophila strain ERS1305867

42.216

88.36

0.373
  pilT Legionella pneumophila strain Lp02

42.216

88.36

0.373
  pilT Pseudomonas aeruginosa PAK

40.988

91.005

0.373
  pilT Pseudomonas stutzeri DSM 10701

40.698

91.005

0.37
  pilT Acinetobacter baumannii strain A118

41.317

88.36

0.365
  pilT Acinetobacter nosocomialis M2

41.317

88.36

0.365
  pilT Acinetobacter baumannii D1279779

41.317

88.36

0.365

Multiple sequence alignment