Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACK2IZ_RS10310 Genome accession   NZ_CP176642
Coordinates   2155696..2156739 (+) Length   347 a.a.
NCBI ID   WP_008791315.1    Uniprot ID   A0A9Q2WWJ4
Organism   Thomasclavelia ramosa strain SFAD15     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2150696..2161739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2IZ_RS10290 - 2151143..2151607 (+) 465 WP_003536122.1 hypothetical protein -
  ACK2IZ_RS10295 - 2151604..2152536 (+) 933 WP_003536123.1 type II secretion system protein -
  ACK2IZ_RS10300 - 2152574..2153998 (+) 1425 WP_003536124.1 type II secretion system protein -
  ACK2IZ_RS10305 - 2154016..2155686 (+) 1671 WP_003536125.1 GspE/PulE family protein -
  ACK2IZ_RS10310 pilT 2155696..2156739 (+) 1044 WP_008791315.1 type IV pilus twitching motility protein PilT Machinery gene
  ACK2IZ_RS10315 - 2156760..2157617 (+) 858 WP_003536127.1 type II secretion system protein -
  ACK2IZ_RS10320 - 2157757..2158947 (+) 1191 WP_003536128.1 ATP-grasp domain-containing protein -
  ACK2IZ_RS10325 - 2158947..2159684 (+) 738 WP_003536129.1 esterase family protein -
  ACK2IZ_RS10330 - 2159697..2160902 (+) 1206 WP_003536130.1 acetyl-CoA carboxylase biotin carboxylase subunit family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38957.86 Da        Isoelectric Point: 6.2847

>NTDB_id=1081848 ACK2IZ_RS10310 WP_008791315.1 2155696..2156739(+) (pilT) [Thomasclavelia ramosa strain SFAD15]
MINEILKASRNNKCSDIHISANTGIKVRQDGLLADYPIEFPQNELIKMIMELIPKRLIDNVDRHEDADFVYHVGEERYRV
NIYYEQTNICAAIRVINDEILTLEQLEMPTVLNQIAMEPRGLVLVTGPTGSGKSTTLAAMIDLVNKQRNCHILTIEDPIE
YVYKQKQALIHQREIGEDVKDFDMALRSAMREDPDVILVGEMRDYETISAVITLAETGHLVFSTLHTIGAAKTIDRIIDI
FPQHKQAQVRTQLSGVLNAVITQTLLPHASGIGRVAAVEIMRANDAVRNLIRDNKGHQINSVIQTGKKEGMISLNHALAN
LVREGKITLDTAKKCASDISEFKQYLQ

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1081848 ACK2IZ_RS10310 WP_008791315.1 2155696..2156739(+) (pilT) [Thomasclavelia ramosa strain SFAD15]
ATGATAAATGAAATTTTAAAAGCTTCTCGAAATAATAAATGTTCAGATATTCATATTAGTGCTAATACAGGAATAAAGGT
TCGTCAGGATGGTTTATTGGCAGATTATCCAATTGAGTTTCCTCAAAATGAATTAATCAAAATGATTATGGAGTTAATTC
CAAAACGATTGATTGATAATGTTGATCGTCATGAAGATGCAGATTTTGTCTATCATGTTGGGGAGGAACGTTATCGGGTC
AATATTTATTATGAACAAACTAATATTTGTGCTGCAATTAGGGTTATTAATGATGAAATTTTAACATTAGAGCAATTAGA
AATGCCCACCGTGTTAAATCAGATTGCAATGGAACCAAGAGGCTTAGTTTTAGTAACAGGACCGACAGGAAGTGGTAAAT
CAACTACTCTAGCAGCAATGATTGATTTAGTGAATAAACAGAGAAATTGTCATATTTTAACAATTGAAGATCCAATTGAA
TATGTTTATAAACAGAAACAAGCATTAATTCATCAACGTGAAATTGGTGAAGATGTAAAAGATTTTGATATGGCATTACG
CTCTGCTATGCGTGAAGACCCGGATGTTATTTTAGTTGGTGAAATGCGTGATTACGAAACAATCAGTGCCGTTATTACAT
TAGCCGAAACTGGTCATTTAGTTTTCTCAACGTTGCACACGATTGGGGCAGCTAAGACAATTGATCGAATAATTGATATT
TTTCCTCAGCATAAACAGGCTCAAGTTAGAACTCAATTATCAGGTGTTTTAAATGCGGTAATTACACAAACTTTATTACC
TCATGCAAGCGGAATAGGACGAGTTGCTGCGGTAGAAATAATGCGTGCTAATGATGCAGTTAGAAATTTAATTCGTGATA
ATAAAGGCCACCAGATCAATTCTGTTATTCAAACGGGTAAAAAAGAAGGGATGATATCTTTAAATCATGCTCTTGCAAAT
CTAGTGCGTGAAGGAAAAATAACTTTAGATACAGCTAAAAAGTGTGCTAGTGATATTTCAGAGTTTAAACAGTATTTACA
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

48.986

99.424

0.487
  pilT Legionella pneumophila strain ERS1305867

48.116

99.424

0.478
  pilT Legionella pneumophila strain Lp02

48.116

99.424

0.478
  pilT Neisseria meningitidis 8013

48.529

97.983

0.476
  pilT Acinetobacter nosocomialis M2

47.536

99.424

0.473
  pilT Acinetobacter baumannii D1279779

47.536

99.424

0.473
  pilT Acinetobacter baumannii strain A118

47.536

99.424

0.473
  pilT Pseudomonas stutzeri DSM 10701

47.536

99.424

0.473
  pilT Neisseria gonorrhoeae MS11

48.235

97.983

0.473
  pilT Pseudomonas aeruginosa PAK

47.788

97.695

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.085

94.524

0.464
  pilT Vibrio cholerae strain A1552

49.085

94.524

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.264

100

0.464

Multiple sequence alignment