Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFWHB_RS08340 Genome accession   NZ_CP172298
Coordinates   1763552..1764691 (-) Length   379 a.a.
NCBI ID   WP_139751448.1    Uniprot ID   -
Organism   Aeromonas sp. A-8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1758552..1769691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFWHB_RS08315 (ACFWHB_08315) - 1758587..1758916 (-) 330 WP_163149208.1 translation initiation factor -
  ACFWHB_RS08320 (ACFWHB_08320) - 1759036..1759263 (+) 228 WP_163149209.1 FeoA family protein -
  ACFWHB_RS08325 (ACFWHB_08325) feoB 1759302..1761572 (+) 2271 WP_395949925.1 Fe(2+) transporter permease subunit FeoB -
  ACFWHB_RS08330 (ACFWHB_08330) - 1761616..1761858 (+) 243 WP_106886441.1 FeoC-like transcriptional regulator -
  ACFWHB_RS08335 (ACFWHB_08335) glgC 1761960..1763231 (-) 1272 WP_111912664.1 glucose-1-phosphate adenylyltransferase -
  ACFWHB_RS08340 (ACFWHB_08340) pilU 1763552..1764691 (-) 1140 WP_139751448.1 type IVa pilus ATPase TapW Machinery gene
  ACFWHB_RS08345 (ACFWHB_08345) - 1764747..1765103 (-) 357 WP_010675200.1 DMT family protein -
  ACFWHB_RS08350 (ACFWHB_08350) - 1765216..1765965 (+) 750 WP_395949926.1 substrate-binding periplasmic protein -
  ACFWHB_RS08355 (ACFWHB_08355) btuC 1766052..1767059 (+) 1008 WP_371161391.1 vitamin B12 ABC transporter permease BtuC -
  ACFWHB_RS08360 (ACFWHB_08360) - 1767046..1767831 (+) 786 WP_395949927.1 ATP-binding cassette domain-containing protein -
  ACFWHB_RS08365 (ACFWHB_08365) - 1768025..1769089 (-) 1065 WP_106886447.1 3-deoxy-7-phosphoheptulonate synthase -
  ACFWHB_RS08370 (ACFWHB_08370) - 1769231..1769611 (+) 381 WP_106886448.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42285.28 Da        Isoelectric Point: 6.4280

>NTDB_id=1066104 ACFWHB_RS08340 WP_139751448.1 1763552..1764691(-) (pilU) [Aeromonas sp. A-8]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALSPLNEVPLEPGEVARIAAELMDSEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEVMLGTHTIRDLIKRGEFGGLKEIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWREKGQIGNSAAASGWSLEPTKDDNNDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1066104 ACFWHB_RS08340 WP_139751448.1 1763552..1764691(-) (pilU) [Aeromonas sp. A-8]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTCTCCACAGGGGCGCCCCCCTG
CGCCAAGTTCAACGGCGCCCTTAGCCCCCTGAACGAGGTGCCGCTCGAGCCGGGCGAGGTGGCCAGGATAGCCGCCGAGC
TCATGGACTCGGAGCAGAAGCAGCAGTTCGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAGGTCTCCCTGGTGGCGCGCAACATCAAGACAGAGATCCCCCGCTTCGA
GGACTTGAAGCTTCCCCCGGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCCACCTCGCTCGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGTGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTCGGAGTGGATACCCGCAGCTTCCACGC
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGTGAAATTCGCGACCGGGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCAGGCCCCAATTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
GCTGGTGAAGACCACAGACGGCGGCCGCCGGGCGGCGGTGGAGGTCATGCTCGGCACCCACACCATTCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGATCATGGAGAAATCCAGGGCCCTTGGCATGGTCACCTTCGACAGCACCCTG
TTCGATCTGGTGGTGGAGGGGGTGATAGACGAAGAAGAGGCGGTCAAGAACGCAGACTCCCAGAACAACCTGCGCCTCAA
GATCAAGCTGTGGCGGGAGAAGGGACAGATAGGCAACAGTGCGGCGGCCAGCGGCTGGAGCCTGGAGCCCACCAAGGATG
ATAACAACGATCTCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.667

98.945

0.541
  pilU Acinetobacter baylyi ADP1

52.676

93.668

0.493
  pilU Vibrio cholerae strain A1552

49.858

93.14

0.464
  pilT Vibrio cholerae strain A1552

40.456

92.612

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

92.612

0.375
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

89.446

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361

Multiple sequence alignment