Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACHMXA_RS00315 Genome accession   NZ_CP171978
Coordinates   72860..73996 (+) Length   378 a.a.
NCBI ID   WP_176668403.1    Uniprot ID   A0A7Y7IBT8
Organism   Variovorax sp. UC74_104     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 67860..78996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXA_RS00290 (ACHMXA_00290) - 68239..69513 (-) 1275 WP_395317588.1 Glu/Leu/Phe/Val dehydrogenase -
  ACHMXA_RS00295 (ACHMXA_00295) - 69799..70224 (+) 426 WP_395317589.1 hypothetical protein -
  ACHMXA_RS00300 (ACHMXA_00300) - 70363..70830 (+) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ACHMXA_RS00305 (ACHMXA_00305) - 70942..71628 (-) 687 WP_395321180.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHMXA_RS00310 (ACHMXA_00310) pilT 71680..72723 (+) 1044 WP_126025783.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHMXA_RS00315 (ACHMXA_00315) pilU 72860..73996 (+) 1137 WP_176668403.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHMXA_RS00320 (ACHMXA_00320) - 74070..74978 (+) 909 WP_176668404.1 NAD(P)-dependent oxidoreductase -
  ACHMXA_RS00325 (ACHMXA_00325) - 75033..75851 (-) 819 WP_395317590.1 BON domain-containing protein -
  ACHMXA_RS00330 (ACHMXA_00330) - 75848..76459 (-) 612 WP_395317591.1 SIS domain-containing protein -
  ACHMXA_RS00335 (ACHMXA_00335) - 76546..76923 (-) 378 WP_176668407.1 YraN family protein -
  ACHMXA_RS00340 (ACHMXA_00340) rsmI 76997..77950 (+) 954 WP_395317592.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACHMXA_RS00345 (ACHMXA_00345) - 77947..78675 (-) 729 WP_395317593.1 glutathione S-transferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41780.94 Da        Isoelectric Point: 6.4178

>NTDB_id=1064707 ACHMXA_RS00315 WP_176668403.1 72860..73996(+) (pilU) [Variovorax sp. UC74_104]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAPHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKEVSMTKRGLCILVGATGSGKSTTLAAMIDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGLGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMKKSRNLG
MQTFDQALFDLFESHSISFEDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1064707 ACHMXA_RS00315 WP_176668403.1 72860..73996(+) (pilU) [Variovorax sp. UC74_104]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACCGCCGACTTTCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTCTCGCAGCAGGCGCTGGGCGCGCCGCACA
CGCTGGCGCTGACCCGCTCGATCATGAACGACCGCCAGACGGCCGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCCACCGGCATCGGCCGCTTCCGCGTGAACGCGTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTGAAGGAGGTGTCGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTGGCGCAACGAGAATTCCTTCGGC
CACATCGTGACGGTCGAAGACCCGGTCGAATTCGTGCATCCGCACAAGAACTGCGTGGTGACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCGGTGGCCTTCGCCGAAACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTCGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGTTCGCTGGTCTCCCAGCGGCTGGTGCCGACCGAAGACGGCCTGGGCCGCGTGGCCGCGGTCGAAGTGCTGCTGAACA
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACATTCGACCAGGCGCTGTTCGACCTCTTCGAGAGCCACTCGATCAGCTTCGAGGACGCCATCCGCAATGCCGA
CTCGGCCAACGACCTTCGCCTGCAGATCAAGCTCAACAGCCAGCGTGCACGCAGCACCGACCTGGCTGCGGGCACGGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(100-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y7IBT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.897

92.328

0.59
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

89.153

0.402
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter nosocomialis M2

43.544

88.095

0.384
  pilT Acinetobacter baumannii strain A118

43.544

88.095

0.384
  pilT Acinetobacter baumannii D1279779

43.544

88.095

0.384
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Legionella pneumophila strain ERS1305867

44.073

87.037

0.384
  pilT Legionella pneumophila strain Lp02

44.073

87.037

0.384
  pilT Acinetobacter baylyi ADP1

42.042

88.095

0.37

Multiple sequence alignment