Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACHMXA_RS00310 Genome accession   NZ_CP171978
Coordinates   71680..72723 (+) Length   347 a.a.
NCBI ID   WP_126025783.1    Uniprot ID   A0A3S0XJW7
Organism   Variovorax sp. UC74_104     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 66680..77723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXA_RS00280 (ACHMXA_00280) - 66956..67618 (-) 663 WP_395317586.1 nucleoside/nucleotide kinase family protein -
  ACHMXA_RS00285 (ACHMXA_00285) - 67683..68204 (-) 522 WP_395317587.1 alpha-ketoglutarate-dependent dioxygenase AlkB family protein -
  ACHMXA_RS00290 (ACHMXA_00290) - 68239..69513 (-) 1275 WP_395317588.1 Glu/Leu/Phe/Val dehydrogenase -
  ACHMXA_RS00295 (ACHMXA_00295) - 69799..70224 (+) 426 WP_395317589.1 hypothetical protein -
  ACHMXA_RS00300 (ACHMXA_00300) - 70363..70830 (+) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ACHMXA_RS00305 (ACHMXA_00305) - 70942..71628 (-) 687 WP_395321180.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACHMXA_RS00310 (ACHMXA_00310) pilT 71680..72723 (+) 1044 WP_126025783.1 type IV pilus twitching motility protein PilT Machinery gene
  ACHMXA_RS00315 (ACHMXA_00315) pilU 72860..73996 (+) 1137 WP_176668403.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACHMXA_RS00320 (ACHMXA_00320) - 74070..74978 (+) 909 WP_176668404.1 NAD(P)-dependent oxidoreductase -
  ACHMXA_RS00325 (ACHMXA_00325) - 75033..75851 (-) 819 WP_395317590.1 BON domain-containing protein -
  ACHMXA_RS00330 (ACHMXA_00330) - 75848..76459 (-) 612 WP_395317591.1 SIS domain-containing protein -
  ACHMXA_RS00335 (ACHMXA_00335) - 76546..76923 (-) 378 WP_176668407.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38178.86 Da        Isoelectric Point: 6.8356

>NTDB_id=1064706 ACHMXA_RS00310 WP_126025783.1 71680..72723(+) (pilT) [Variovorax sp. UC74_104]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVNGDVRRINVDALDHKGVHAMVYDIMSDTHRKHYEEFLEVDFSFEIDGLAR
FRVNAFNQARGAAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMINYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFSNALRSALREDPDAILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGEEKEMIRAMLSESLQAVISQTLCKTKDGQSRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTGQGSGMQTLDQN
LTELVRRNTISAAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1064706 ACHMXA_RS00310 WP_126025783.1 71680..72723(+) (pilT) [Variovorax sp. UC74_104]
GTGGACATCACCCAACTGCTGGCGTTCAGCGTCAAGAACAAAGCCTCCGACCTGCACCTTTCCGCGGGCCTGCCGCCCAT
GATCCGCGTCAACGGCGACGTGCGCCGCATCAACGTGGACGCGCTCGACCACAAGGGCGTGCACGCCATGGTGTACGACA
TCATGAGCGACACGCACCGCAAGCACTACGAAGAGTTCCTGGAGGTCGACTTCTCCTTCGAGATCGACGGCCTCGCGCGC
TTCCGCGTGAACGCCTTCAACCAGGCGCGCGGCGCGGCGGCGGTGTTCCGGACCATTCCCTCGAAGATCCTCACGCTCGA
GCAGCTCAACGCGCCGAAGATTTTCGGCGAACTGGCGCTCAAGCCGCGCGGGCTGGTTCTGGTGACGGGCCCCACGGGCT
CGGGCAAGTCGACCACGCTGGCGGCCATGATCAACTACCTCAACGAGAACGAGTACGGCCACATCCTCACGGTGGAAGAC
CCGATCGAGTTCGTGCACGAGTCGAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGATGACGCTGTCGTTCTCCAA
CGCCCTGCGCTCCGCCCTGCGCGAAGACCCTGACGCCATCCTGGTGGGCGAGCTGCGCGACCTGGAGACCATCCGCCTGG
CCATGACCGCCGCCGAGACGGGCCACCTGGTGTTCGGCACGCTGCACACCTCTTCGGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCGGGCGAAGAGAAGGAAATGATCCGCGCCATGCTCTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGAGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGCCGGCCATCCGAAACCTCA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCGGCCAGGGCTCTGGCATGCAGACGCTGGACCAGAAC
CTGACGGAACTGGTGCGCCGCAATACCATCTCGGCAGCAGAAGCCCGAGGAAAAGCCAAGATCCCAGAGAATTTCCCGGG
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S0XJW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.652

99.424

0.752
  pilT Acinetobacter nosocomialis M2

75.362

99.424

0.749
  pilT Acinetobacter baumannii D1279779

75.362

99.424

0.749
  pilT Acinetobacter baumannii strain A118

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.328

98.271

0.671
  pilT Vibrio cholerae strain A1552

68.328

98.271

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

43.881

96.542

0.424
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398

Multiple sequence alignment