Detailed information NTG1064278

Bioinformatically predicted

Overview

• Name: pilA/pilAI
• Type: Machinery gene
• Locus tag: ACHFCC_RS00500
• Genome accession: NZ_CP171806
• Coordinates: 103939..104343 (-)
• Length: 134 a.a.
• NCBI ID: WP_395139964.1
• Uniprot ID: -
• Organism: Schlegelella aquatica strain HS-12-14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 98939..109343

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACHFCC_RS00475 (ACHFCC_00475)	-	98948..100402 (-)	1455	WP_395142590.1	M48 family metalloprotease	-
ACHFCC_RS00480 (ACHFCC_00480)	moaC	100729..101232 (+)	504	WP_395139956.1	cyclic pyranopterin monophosphate synthase MoaC	-
ACHFCC_RS00485 (ACHFCC_00485)	-	101257..102909 (-)	1653	WP_395139958.1	Wzy polymerase domain-containing protein	-
ACHFCC_RS00490 (ACHFCC_00490)	-	102974..103195 (+)	222	WP_395139960.1	hypothetical protein	-
ACHFCC_RS00495 (ACHFCC_00495)	-	103458..103862 (-)	405	WP_395139962.1	pilin	-
ACHFCC_RS00500 (ACHFCC_00500)	pilA/pilAI	103939..104343 (-)	405	WP_395139964.1	pilin	Machinery gene
ACHFCC_RS00505 (ACHFCC_00505)	-	104531..105061 (-)	531	WP_395139966.1	hypothetical protein	-
ACHFCC_RS00510 (ACHFCC_00510)	-	105173..105427 (+)	255	WP_019561025.1	Arc family DNA-binding protein	-
ACHFCC_RS00515 (ACHFCC_00515)	-	105424..105846 (+)	423	WP_395139969.1	type II toxin-antitoxin system VapC family toxin	-
ACHFCC_RS00520 (ACHFCC_00520)	-	105936..106178 (+)	243	WP_019561027.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
ACHFCC_RS00525 (ACHFCC_00525)	-	106175..106570 (+)	396	WP_395139971.1	PIN domain-containing protein	-
ACHFCC_RS00530 (ACHFCC_00530)	-	106611..106895 (-)	285	WP_395142592.1	addiction module antidote protein	-
ACHFCC_RS00535 (ACHFCC_00535)	-	107151..107432 (-)	282	WP_026330211.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ACHFCC_RS00540 (ACHFCC_00540)	-	107510..108619 (-)	1110	WP_395139973.1	alkene reductase	-

Sequence

Protein

• Length: 134 a.a.
• Molecular weight: 14125.46 Da
• Isoelectric Point: 8.4815

>NTDB_id=1064278 ACHFCC_RS00500 WP_395139964.1 103939..104343(-) (pilA/pilAI) [Schlegelella aquatica strain HS-12-14]
MKRVQQGFTLIELMIVVAIIGILAAIGLPMYQDYVAKSQVTAGLAEITPGKTQYEVLLNEGKTTITGISELGLKNSDRCT
ITPITALSGTGTIVCTLKGNPKVANQTITLTRATDGTWSCSTTVDAKYKPAGCS

Nucleotide

• Length: 405 bp

>NTDB_id=1064278 ACHFCC_RS00500 WP_395139964.1 103939..104343(-) (pilA/pilAI) [Schlegelella aquatica strain HS-12-14]
ATGAAGCGTGTTCAGCAGGGTTTCACCCTCATCGAACTGATGATCGTCGTGGCGATCATCGGTATCCTGGCCGCCATCGG
GCTGCCGATGTACCAGGACTATGTCGCCAAGTCGCAAGTCACCGCTGGTCTCGCCGAGATCACTCCCGGCAAGACTCAGT
ATGAAGTTCTGCTCAACGAGGGCAAAACCACCATCACCGGCATCAGCGAACTCGGCCTGAAGAACTCTGACCGCTGCACG
ATCACCCCCATTACGGCTCTGTCTGGAACGGGCACTATCGTGTGCACCTTGAAGGGCAACCCGAAGGTGGCGAATCAAAC
CATCACGCTTACTCGCGCGACCGACGGAACCTGGTCGTGCTCGACGACCGTCGACGCGAAGTACAAGCCTGCCGGCTGCA
GCTGA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (36-133)

• Prokaryotic N-terminal methylation motif (N_methyl) (3-27)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA/pilAI	Pseudomonas stutzeri DSM 10701	55.072	100	0.567
pilA	Acinetobacter baumannii strain A118	51.825	100	0.53
pilA/pilAII	Pseudomonas stutzeri DSM 10701	46.853	100	0.5
pilA	Pseudomonas aeruginosa PAK	40.541	100	0.448
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	41.958	100	0.448
pilA	Vibrio cholerae strain A1552	41.958	100	0.448
pilA	Vibrio cholerae C6706	41.958	100	0.448
pilA2	Legionella pneumophila str. Paris	39.333	100	0.44
pilA	Ralstonia pseudosolanacearum GMI1000	34.32	100	0.433
pilA2	Legionella pneumophila strain ERS1305867	38.667	100	0.433
comP	Acinetobacter baylyi ADP1	40.278	100	0.433
pilA	Vibrio parahaemolyticus RIMD 2210633	39.13	100	0.403