Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   ACF3NW_RS12225 Genome accession   NZ_CP171110
Coordinates   2398769..2399218 (+) Length   149 a.a.
NCBI ID   WP_314251887.1    Uniprot ID   -
Organism   Eikenella halliae strain WGS1533     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2393769..2404218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3NW_RS12195 (ACF3NW_12195) - 2393910..2394305 (-) 396 WP_392423404.1 energy transducer TonB -
  ACF3NW_RS12200 (ACF3NW_12200) orn 2394477..2395064 (-) 588 WP_314251897.1 oligoribonuclease -
  ACF3NW_RS12205 (ACF3NW_12205) - 2395192..2395830 (+) 639 WP_314251895.1 MBL fold metallo-hydrolase -
  ACF3NW_RS12210 (ACF3NW_12210) - 2396151..2396588 (+) 438 WP_314251893.1 MliC family protein -
  ACF3NW_RS12215 (ACF3NW_12215) tsaA 2396910..2397596 (+) 687 WP_314251891.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  ACF3NW_RS12220 (ACF3NW_12220) pilA/pilA1 2398239..2398703 (+) 465 WP_392423405.1 pilin Machinery gene
  ACF3NW_RS12225 (ACF3NW_12225) pilA/pilA1 2398769..2399218 (+) 450 WP_314251887.1 pilin Machinery gene
  ACF3NW_RS12230 (ACF3NW_12230) tfpZ 2399255..2399992 (+) 738 WP_392423406.1 TfpX/TfpZ family type IV pilin accessory protein -
  ACF3NW_RS12235 (ACF3NW_12235) tehB 2400216..2401079 (+) 864 WP_314251883.1 tellurite resistance methyltransferase TehB -
  ACF3NW_RS12240 (ACF3NW_12240) - 2401463..2403334 (+) 1872 WP_314251881.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15527.84 Da        Isoelectric Point: 4.8776

>NTDB_id=1060132 ACF3NW_RS12225 WP_314251887.1 2398769..2399218(+) (pilA/pilA1) [Eikenella halliae strain WGS1533]
MSRIKGFTLIELMIVIAIIGILAAIALPAYDGYVGRSQAVEGFKLTSGLRDDIASVVADTNAFPTAAMVAPNGAIGSIAS
SLDGKYVQNGGVTVTADTGVITVLFDQGVISGSNMQLIPTFNNTNRTQLIQWQCGGTLNSKYLPSSCQP

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1060132 ACF3NW_RS12225 WP_314251887.1 2398769..2399218(+) (pilA/pilA1) [Eikenella halliae strain WGS1533]
ATGTCAAGAATTAAGGGTTTTACTCTGATTGAACTGATGATAGTTATTGCTATTATTGGCATTCTGGCCGCAATCGCTTT
GCCCGCTTATGATGGTTATGTTGGTCGCAGCCAAGCAGTTGAAGGATTTAAATTAACTAGTGGATTGCGCGATGATATTG
CTTCTGTTGTGGCGGATACAAATGCTTTCCCAACGGCTGCTATGGTTGCACCAAATGGGGCTATCGGATCTATCGCTAGT
AGCTTAGATGGTAAGTACGTGCAAAATGGTGGAGTTACTGTTACCGCAGATACTGGTGTGATTACGGTGCTATTTGATCA
AGGTGTAATTTCTGGTTCAAATATGCAATTAATCCCTACATTTAATAATACAAATAGAACGCAATTAATTCAATGGCAAT
GTGGGGGGACATTAAACAGTAAATATTTGCCATCAAGCTGCCAGCCATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

52.703

99.329

0.523
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.973

100

0.43
  comP Acinetobacter baylyi ADP1

37.821

100

0.396
  pilA2 Legionella pneumophila strain ERS1305867

39.189

99.329

0.389
  pilA2 Legionella pneumophila str. Paris

38.514

99.329

0.383
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.514

99.329

0.383
  pilA Vibrio cholerae C6706

38.514

99.329

0.383
  pilA Vibrio cholerae strain A1552

38.514

99.329

0.383
  pilE Neisseria gonorrhoeae strain FA1090

34.132

100

0.383
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.601

100

0.376
  pilE Neisseria gonorrhoeae MS11

33.333

100

0.369
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.486

99.329

0.362

Multiple sequence alignment