Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFJI2_RS00885 Genome accession   NZ_CP170549
Coordinates   197708..198844 (-) Length   378 a.a.
NCBI ID   WP_132136366.1    Uniprot ID   -
Organism   Pseudoduganella sp. UC29_71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 192708..203844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJI2_RS00870 (ACFJI2_00870) - 192759..193754 (-) 996 WP_107877291.1 two-component system response regulator -
  ACFJI2_RS00875 (ACFJI2_00875) - 193751..197170 (-) 3420 WP_377703345.1 MHYT domain-containing protein -
  ACFJI2_RS00880 (ACFJI2_00880) - 197214..197699 (-) 486 WP_377703346.1 glutathione peroxidase -
  ACFJI2_RS00885 (ACFJI2_00885) pilU 197708..198844 (-) 1137 WP_132136366.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFJI2_RS00890 (ACFJI2_00890) pilT 198914..199957 (-) 1044 WP_107877283.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFJI2_RS00895 (ACFJI2_00895) - 200026..200748 (+) 723 WP_377703347.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFJI2_RS00900 (ACFJI2_00900) proC 200758..201672 (+) 915 WP_377703348.1 pyrroline-5-carboxylate reductase -
  ACFJI2_RS00905 (ACFJI2_00905) - 201838..202191 (-) 354 WP_377703349.1 YqjD family protein -
  ACFJI2_RS00910 (ACFJI2_00910) ubiA 202249..203103 (-) 855 WP_377703350.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41906.34 Da        Isoelectric Point: 6.4216

>NTDB_id=1057907 ACFJI2_RS00885 WP_132136366.1 197708..198844(-) (pilU) [Pseudoduganella sp. UC29_71]
MERDQASKFMFDLLRLMVSKNGSDLFITAGFPPAMKIDGKMTPVSAQPLTAAHTSDLARSIMNDKQTAGFELTKEANFAI
SPGDLGRFRVSAFVQMSAVGMVLRTINSAIPKLEDLGLPEVLKDVVMSKRGLVIMVGATGSGKSTTLAAMVGYRNENSYG
HIITIEDPVEFVHPHRNCIVTQREVGVDTEDWEVALKNTLRQAPDVIQIGEIRDRQTMDHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPRKETKGRAVAIEIMLNSPLISDLIFKGQVHEIKELMKKSRELG
MQTFDQSLFDLFEADVISYEDALRNADSVNDLRLAIKLEGKAAKTRDLSAGTEHLNII

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1057907 ACFJI2_RS00885 WP_132136366.1 197708..198844(-) (pilU) [Pseudoduganella sp. UC29_71]
ATGGAACGCGACCAGGCCTCAAAATTCATGTTCGACCTGCTGCGCCTGATGGTCAGCAAGAATGGTTCGGATCTGTTCAT
CACGGCCGGCTTTCCGCCGGCGATGAAAATCGACGGCAAGATGACGCCGGTGTCGGCCCAGCCGCTGACGGCGGCCCACA
CCTCGGACCTGGCCCGCTCCATCATGAACGACAAGCAGACCGCCGGCTTCGAGCTGACCAAGGAGGCCAACTTCGCCATC
AGCCCCGGCGACCTGGGACGCTTCCGCGTCTCGGCCTTCGTGCAGATGAGCGCCGTGGGCATGGTGCTGCGCACGATTAA
CAGCGCCATCCCCAAGCTGGAGGACCTGGGCCTGCCGGAAGTGCTGAAGGACGTGGTGATGTCCAAGCGCGGCCTGGTGA
TCATGGTGGGCGCCACCGGCTCGGGCAAATCGACCACGCTGGCGGCCATGGTGGGCTACCGCAACGAGAACAGCTACGGC
CACATCATCACCATCGAGGACCCGGTGGAATTCGTGCACCCGCACCGCAACTGCATCGTCACGCAGCGCGAAGTGGGCGT
GGACACGGAGGACTGGGAGGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCCAGACCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAACGCCGCCAGCAGCTGCTGATGGATTTGTCGCTCAACCT
GAAGGGCATGATTTCGCAGCGCCTGATCCCGCGCAAGGAAACCAAGGGCCGCGCGGTGGCCATCGAGATCATGCTCAATT
CGCCGCTGATTTCGGACCTGATCTTCAAGGGCCAGGTGCACGAGATCAAGGAGCTGATGAAAAAATCGCGCGAGCTGGGC
ATGCAGACTTTCGACCAGTCGCTGTTCGACCTGTTCGAGGCGGACGTGATCAGCTACGAGGACGCGCTGCGCAACGCCGA
TTCCGTCAACGACCTGCGCCTGGCCATCAAGCTCGAAGGCAAGGCCGCCAAGACGCGCGACCTGTCGGCCGGCACCGAAC
ACCTGAACATCATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(70-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.74

91.534

0.593
  pilU Acinetobacter baylyi ADP1

58.543

94.444

0.553
  pilU Vibrio cholerae strain A1552

57.478

90.212

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.582

88.624

0.386
  pilT Pseudomonas aeruginosa PAK

43.284

88.624

0.384
  pilT Acinetobacter nosocomialis M2

42.9

87.566

0.376
  pilT Acinetobacter baumannii D1279779

42.9

87.566

0.376
  pilT Acinetobacter baumannii strain A118

42.9

87.566

0.376
  pilT Acinetobacter baylyi ADP1

42.296

87.566

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.624

0.368
  pilT Legionella pneumophila strain Lp02

41.692

87.566

0.365
  pilT Legionella pneumophila strain ERS1305867

41.692

87.566

0.365

Multiple sequence alignment