Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACDW36_RS25135 Genome accession   NZ_CP167984
Coordinates   5394704..5395156 (-) Length   150 a.a.
NCBI ID   WP_031636800.1    Uniprot ID   P17837
Organism   Pseudomonas aeruginosa strain 0304d2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5389704..5400156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW36_RS25115 (ACDW36_25115) ampD 5390399..5390965 (-) 567 WP_023096121.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACDW36_RS25120 (ACDW36_25120) - 5391116..5393371 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  ACDW36_RS25125 (ACDW36_25125) nadC 5393657..5394505 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACDW36_RS25135 (ACDW36_25135) pilA 5394704..5395156 (-) 453 WP_031636800.1 pilin Machinery gene
  ACDW36_RS25140 (ACDW36_25140) pilB 5395388..5397088 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACDW36_RS25145 (ACDW36_25145) pilC 5397092..5398312 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  ACDW36_RS25150 (ACDW36_25150) pilD 5398316..5399188 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACDW36_RS25155 (ACDW36_25155) coaE 5399185..5399796 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ACDW36_RS25160 (ACDW36_25160) yacG 5399793..5399993 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15743.11 Da        Isoelectric Point: 8.4742

>NTDB_id=1040789 ACDW36_RS25135 WP_031636800.1 5394704..5395156(-) (pilA) [Pseudomonas aeruginosa strain 0304d2]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVTIKDTGDGTVKFTFATGQSSPKNAGKEITLNRTAEGVWTCTSTQEEMFIPKGCNKP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=1040789 ACDW36_RS25135 WP_031636800.1 5394704..5395156(-) (pilA) [Pseudomonas aeruginosa strain 0304d2]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTGTAAAATTTAC
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAAGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATAAGCCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P17837

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

66.447

100

0.673
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45

100

0.48
  pilA Vibrio cholerae strain A1552

45

100

0.48
  pilA Vibrio cholerae C6706

45

100

0.48
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46

100

0.46
  pilA Acinetobacter baumannii strain A118

45.27

98.667

0.447
  pilA Vibrio parahaemolyticus RIMD 2210633

42.568

98.667

0.42
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

95.333

0.413
  comP Acinetobacter baylyi ADP1

41.333

100

0.413
  pilA/pilA1 Eikenella corrodens VA1

39.865

98.667

0.393
  pilA2 Legionella pneumophila str. Paris

38.255

99.333

0.38
  pilA2 Legionella pneumophila strain ERS1305867

38.255

99.333

0.38

Multiple sequence alignment