Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACDW36_RS25145 Genome accession   NZ_CP167984
Coordinates   5397092..5398312 (+) Length   406 a.a.
NCBI ID   WP_009315898.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 0304d2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5392092..5403312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDW36_RS25125 (ACDW36_25125) nadC 5393657..5394505 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACDW36_RS25135 (ACDW36_25135) pilA 5394704..5395156 (-) 453 WP_031636800.1 pilin Machinery gene
  ACDW36_RS25140 (ACDW36_25140) pilB 5395388..5397088 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACDW36_RS25145 (ACDW36_25145) pilC 5397092..5398312 (+) 1221 WP_009315898.1 type 4a pilus biogenesis protein PilC Machinery gene
  ACDW36_RS25150 (ACDW36_25150) pilD 5398316..5399188 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACDW36_RS25155 (ACDW36_25155) coaE 5399185..5399796 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ACDW36_RS25160 (ACDW36_25160) yacG 5399793..5399993 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  ACDW36_RS25165 (ACDW36_25165) - 5400030..5400239 (-) 210 WP_003094660.1 hypothetical protein -
  ACDW36_RS25170 (ACDW36_25170) - 5400345..5401034 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  ACDW36_RS25175 (ACDW36_25175) - 5401031..5401501 (-) 471 WP_023096122.1 hypothetical protein -
  ACDW36_RS25180 (ACDW36_25180) - 5401498..5401923 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  ACDW36_RS25185 (ACDW36_25185) - 5402056..5402685 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  ACDW36_RS25190 (ACDW36_25190) - 5402682..5403131 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44288.04 Da        Isoelectric Point: 9.8784

>NTDB_id=1040791 ACDW36_RS25145 WP_009315898.1 5397092..5398312(+) (pilC) [Pseudomonas aeruginosa strain 0304d2]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGAIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1040791 ACDW36_RS25145 WP_009315898.1 5397092..5398312(+) (pilC) [Pseudomonas aeruginosa strain 0304d2]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGCTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCGCAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGTGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAGAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAATTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGTGTGGGGGCGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCAGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGCCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baumannii D1279779

60.891

99.507

0.606
  pilC Acinetobacter baylyi ADP1

60.591

100

0.606
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.562

97.783

0.406
  pilC Thermus thermophilus HB27

37.157

98.768

0.367

Multiple sequence alignment