Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABL843_RS30665 Genome accession   NZ_CP157620
Coordinates   6718861..6719304 (-) Length   147 a.a.
NCBI ID   WP_418118482.1    Uniprot ID   -
Organism   Variovorax sp. 350MFTsu5.1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6713861..6724304
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL843_RS30650 (ABL843_30645) - 6714529..6715764 (-) 1236 WP_418118479.1 glycosyltransferase -
  ABL843_RS30655 (ABL843_30650) - 6715797..6716783 (-) 987 WP_418118480.1 glycosyltransferase family 2 protein -
  ABL843_RS30660 (ABL843_30655) - 6716794..6718791 (-) 1998 WP_418118481.1 hypothetical protein -
  ABL843_RS30665 (ABL843_30660) pilA 6718861..6719304 (-) 444 WP_418118482.1 pilin Machinery gene
  ABL843_RS30670 (ABL843_30665) - 6719519..6720322 (-) 804 WP_418118483.1 3',5'-cyclic-nucleotide phosphodiesterase -
  ABL843_RS30675 (ABL843_30670) - 6720352..6722610 (-) 2259 WP_418118484.1 CHASE2 domain-containing protein -
  ABL843_RS30680 (ABL843_30675) - 6722662..6723315 (-) 654 WP_126021038.1 FHA domain-containing protein -
  ABL843_RS30685 (ABL843_30680) - 6723388..6724197 (-) 810 WP_126021037.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 14950.28 Da        Isoelectric Point: 10.5062

>NTDB_id=1007772 ABL843_RS30665 WP_418118482.1 6718861..6719304(-) (pilA) [Variovorax sp. 350MFTsu5.1]
MNRRTLARRAQAGFTLIELMIVVAIIGILAAVALPAYQDYTKRARVSEGLGLAAAAKAGVGEYFASKAAWPTSNASAGLP
AAASIKGNAVNTVTVGTAGVITIAYNTLVVSGGTVTLTPSSANAGSVKWTCKGGSTFPTKYLPSECR

Nucleotide


Download         Length: 444 bp        

>NTDB_id=1007772 ABL843_RS30665 WP_418118482.1 6718861..6719304(-) (pilA) [Variovorax sp. 350MFTsu5.1]
ATGAACCGTCGTACTCTCGCCCGCCGCGCTCAGGCCGGCTTTACCCTTATCGAACTGATGATCGTTGTGGCGATCATTGG
TATCTTGGCTGCGGTGGCCCTGCCGGCGTATCAGGACTACACGAAGCGCGCTCGCGTTTCGGAAGGCCTAGGTCTGGCTG
CCGCTGCCAAGGCTGGTGTGGGTGAATACTTCGCGTCCAAGGCTGCTTGGCCGACGAGCAACGCCTCTGCCGGATTGCCG
GCGGCTGCCTCGATTAAGGGTAACGCTGTGAATACCGTTACGGTCGGCACCGCTGGCGTGATTACCATCGCCTACAACAC
GCTGGTCGTTAGCGGGGGGACCGTCACGTTGACACCGTCGAGTGCCAACGCTGGTAGCGTCAAGTGGACATGCAAGGGTG
GTAGCACTTTCCCAACGAAGTACTTGCCGTCGGAATGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

45.087

100

0.531
  pilA2 Legionella pneumophila strain ERS1305867

53.285

93.197

0.497
  pilA2 Legionella pneumophila str. Paris

53.285

93.197

0.497
  pilE Neisseria gonorrhoeae MS11

45.86

100

0.49
  pilE Neisseria gonorrhoeae strain FA1090

45.161

100

0.476
  comP Acinetobacter baylyi ADP1

45.946

100

0.463
  pilA/pilA1 Eikenella corrodens VA1

39.506

100

0.435
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

32.984

100

0.429
  pilA Haemophilus influenzae Rd KW20

39.597

100

0.401
  pilA Pseudomonas aeruginosa PAK

34.375

100

0.374
  pilA Acinetobacter baumannii strain A118

38.732

96.599

0.374
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.298

95.918

0.367
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.552

98.639

0.361

Multiple sequence alignment