Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ABLU29_RS04035 Genome accession   NZ_CP157498
Coordinates   711509..712447 (+) Length   312 a.a.
NCBI ID   WP_058212859.1    Uniprot ID   -
Organism   Lactococcus lactis strain 2B-5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 706509..717447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLU29_RS04030 (ABLU29_04020) - 706737..711389 (+) 4653 WP_406841036.1 PolC-type DNA polymerase III -
  ABLU29_RS04035 (ABLU29_04025) comGA 711509..712447 (+) 939 WP_058212859.1 competence type IV pilus ATPase ComGA Machinery gene
  ABLU29_RS04040 (ABLU29_04030) comGB 712341..713414 (+) 1074 WP_406835453.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABLU29_RS04045 (ABLU29_04035) comGC 713428..713811 (+) 384 WP_010906318.1 competence type IV pilus major pilin ComGC Machinery gene
  ABLU29_RS04050 (ABLU29_04040) comGD 713786..714202 (+) 417 WP_021216554.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABLU29_RS04055 (ABLU29_04045) comGE 714174..714470 (+) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABLU29_RS04060 (ABLU29_04050) comGF 714433..714879 (+) 447 WP_029344525.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABLU29_RS04065 (ABLU29_04055) comGG 714927..715202 (+) 276 WP_257012268.1 hypothetical protein Machinery gene
  ABLU29_RS04070 (ABLU29_04060) - 715283..715720 (+) 438 WP_058213216.1 zinc-dependent MarR family transcriptional regulator -
  ABLU29_RS04075 (ABLU29_04065) - 715717..716559 (+) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35648.90 Da        Isoelectric Point: 6.6176

>NTDB_id=1007226 ABLU29_RS04035 WP_058212859.1 711509..712447(+) (comGA) [Lactococcus lactis strain 2B-5]
MIPKMAKELIQRAIDRGASDIYLIASKEKYKLYFRQMTARDLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKKLDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1007226 ABLU29_RS04035 WP_058212859.1 711509..712447(+) (comGA) [Lactococcus lactis strain 2B-5]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGGTGCATCAGATATTTATCTGATTGCATCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCACGTGATCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAAAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGCAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.923

100

0.769
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.662

99.679

0.545
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.698

99.679

0.535
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.733

99.679

0.526
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.733

99.679

0.526
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.733

99.679

0.526
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.733

99.679

0.526
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.603

100

0.516

Multiple sequence alignment