Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   ABLU29_RS04040 Genome accession   NZ_CP157498
Coordinates   712341..713414 (+) Length   357 a.a.
NCBI ID   WP_406835453.1    Uniprot ID   -
Organism   Lactococcus lactis strain 2B-5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 707341..718414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLU29_RS04035 (ABLU29_04025) comGA 711509..712447 (+) 939 WP_058212859.1 competence type IV pilus ATPase ComGA Machinery gene
  ABLU29_RS04040 (ABLU29_04030) comGB 712341..713414 (+) 1074 WP_406835453.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABLU29_RS04045 (ABLU29_04035) comGC 713428..713811 (+) 384 WP_010906318.1 competence type IV pilus major pilin ComGC Machinery gene
  ABLU29_RS04050 (ABLU29_04040) comGD 713786..714202 (+) 417 WP_021216554.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABLU29_RS04055 (ABLU29_04045) comGE 714174..714470 (+) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABLU29_RS04060 (ABLU29_04050) comGF 714433..714879 (+) 447 WP_029344525.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABLU29_RS04065 (ABLU29_04055) comGG 714927..715202 (+) 276 WP_257012268.1 hypothetical protein Machinery gene
  ABLU29_RS04070 (ABLU29_04060) - 715283..715720 (+) 438 WP_058213216.1 zinc-dependent MarR family transcriptional regulator -
  ABLU29_RS04075 (ABLU29_04065) - 715717..716559 (+) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  ABLU29_RS04080 (ABLU29_04070) - 716736..717473 (+) 738 WP_012898617.1 metal ABC transporter ATP-binding protein -
  ABLU29_RS04085 (ABLU29_04075) - 717466..718275 (+) 810 WP_014570791.1 metal ABC transporter permease -

Sequence

Protein


Download         Length: 357 a.a.        Molecular weight: 40392.58 Da        Isoelectric Point: 10.3000

>NTDB_id=1007227 ABLU29_RS04040 WP_406835453.1 712341..713414(+) (comGB) [Lactococcus lactis strain 2B-5]
MSLGHQKIGIKKLISYLQMDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYVDECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPFYSNMGSGI

Nucleotide


Download         Length: 1074 bp        

>NTDB_id=1007227 ABLU29_RS04040 WP_406835453.1 712341..713414(+) (comGB) [Lactococcus lactis strain 2B-5]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTAAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTTGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCGTTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGTGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTCGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCTTCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-348)

Type II secretion system (T2SS), protein F (T2SSF)

(41-159)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

82.991

95.518

0.793
  comYB Streptococcus gordonii str. Challis substr. CH1

53.666

95.518

0.513
  comYB Streptococcus mutans UA140

52.786

95.518

0.504
  comYB Streptococcus mutans UA159

52.493

95.518

0.501
  comGB/cglB Streptococcus mitis SK321

51.045

93.838

0.479
  comGB/cglB Streptococcus pneumoniae Rx1

50.746

93.838

0.476
  comGB/cglB Streptococcus pneumoniae D39

50.746

93.838

0.476
  comGB/cglB Streptococcus pneumoniae R6

50.746

93.838

0.476
  comGB/cglB Streptococcus pneumoniae TIGR4

50.746

93.838

0.476
  comGB/cglB Streptococcus mitis NCTC 12261

50.149

93.838

0.471

Multiple sequence alignment