Detailed information NTG1007231

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: ABLU29_RS04060
• Genome accession: NZ_CP157498
• Coordinates: 714433..714879 (+)
• Length: 148 a.a.
• NCBI ID: WP_029344525.1
• Uniprot ID: A0AAP4JUI5
• Organism: Lactococcus lactis strain 2B-5
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 709433..719879

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABLU29_RS04035 (ABLU29_04025)	comGA	711509..712447 (+)	939	WP_058212859.1	competence type IV pilus ATPase ComGA	Machinery gene
ABLU29_RS04040 (ABLU29_04030)	comGB	712341..713414 (+)	1074	WP_406835453.1	competence type IV pilus assembly protein ComGB	Machinery gene
ABLU29_RS04045 (ABLU29_04035)	comGC	713428..713811 (+)	384	WP_010906318.1	competence type IV pilus major pilin ComGC	Machinery gene
ABLU29_RS04050 (ABLU29_04040)	comGD	713786..714202 (+)	417	WP_021216554.1	competence type IV pilus minor pilin ComGD	Machinery gene
ABLU29_RS04055 (ABLU29_04045)	comGE	714174..714470 (+)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
ABLU29_RS04060 (ABLU29_04050)	comGF	714433..714879 (+)	447	WP_029344525.1	competence type IV pilus minor pilin ComGF	Machinery gene
ABLU29_RS04065 (ABLU29_04055)	comGG	714927..715202 (+)	276	WP_257012268.1	hypothetical protein	Machinery gene
ABLU29_RS04070 (ABLU29_04060)	-	715283..715720 (+)	438	WP_058213216.1	zinc-dependent MarR family transcriptional regulator	-
ABLU29_RS04075 (ABLU29_04065)	-	715717..716559 (+)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
ABLU29_RS04080 (ABLU29_04070)	-	716736..717473 (+)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
ABLU29_RS04085 (ABLU29_04075)	-	717466..718275 (+)	810	WP_014570791.1	metal ABC transporter permease	-
ABLU29_RS04090 (ABLU29_04080)	-	718314..719180 (-)	867	WP_058213299.1	RluA family pseudouridine synthase	-
ABLU29_RS04095 (ABLU29_04085)	-	719385..719762 (+)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17111.91 Da
• Isoelectric Point: 8.6780

>NTDB_id=1007231 ABLU29_RS04060 WP_029344525.1 714433..714879(+) (comGF) [Lactococcus lactis strain 2B-5]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=1007231 ABLU29_RS04060 WP_029344525.1 714433..714879(+) (comGF) [Lactococcus lactis strain 2B-5]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCAATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	93.919	0.716
comYF	Streptococcus mutans UA140	48.227	95.27	0.459
comYF	Streptococcus mutans UA159	47.518	95.27	0.453
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385