Detailed information NTG1007058

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: ABK819_RS25235
• Genome accession: NZ_CP157460
• Coordinates: 5396009..5396461 (-)
• Length: 150 a.a.
• NCBI ID: WP_031754077.1
• Uniprot ID: P08015
• Organism: Pseudomonas aeruginosa isolate PA103_delta_UT+fleQ
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5391009..5401461

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABK819_RS25215 (ABK819_25190)	ampD	5391704..5392270 (-)	567	WP_003112844.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
ABK819_RS25220 (ABK819_25195)	-	5392421..5394676 (-)	2256	WP_031754075.1	DUF1631 domain-containing protein	-
ABK819_RS25225 (ABK819_25200)	nadC	5394962..5395810 (+)	849	WP_003161760.1	carboxylating nicotinate-nucleotide diphosphorylase	-
ABK819_RS25235 (ABK819_25210)	pilA	5396009..5396461 (-)	453	WP_031754077.1	pilin	Machinery gene
ABK819_RS25240 (ABK819_25215)	pilB	5396693..5398393 (+)	1701	WP_023094327.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ABK819_RS25245 (ABK819_25220)	pilC	5398397..5399617 (+)	1221	WP_003112840.1	type 4a pilus biogenesis protein PilC	Machinery gene
ABK819_RS25250 (ABK819_25225)	pilD	5399621..5400493 (+)	873	WP_019486071.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
ABK819_RS25255 (ABK819_25230)	coaE	5400490..5401101 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
ABK819_RS25260 (ABK819_25235)	yacG	5401098..5401298 (+)	201	WP_019486072.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 150 a.a.
• Molecular weight: 15799.09 Da
• Isoelectric Point: 6.3229

>NTDB_id=1007058 ABK819_RS25235 WP_031754077.1 5396009..5396461(-) (pilA) [Pseudomonas aeruginosa isolate PA103_delta_UT+fleQ]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVTIKDTGDGTIKFNFATGQSSPKNAGREITLNRTAEGVWTCTSTQEEMFIPKGCNEP

Nucleotide

• Length: 453 bp

>NTDB_id=1007058 ABK819_RS25235 WP_031754077.1 5396009..5396461(-) (pilA) [Pseudomonas aeruginosa isolate PA103_delta_UT+fleQ]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTATAAAATTTAA
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAGGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATGAGCCTTAA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (35-148)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-26)

3D structure

• AlphaFold DB: P08015

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	65.132	100	0.66
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	46.622	98.667	0.46
pilA	Vibrio cholerae strain A1552	46.622	98.667	0.46
pilA	Vibrio cholerae C6706	46.622	98.667	0.46
pilA/pilAI	Pseudomonas stutzeri DSM 10701	45.333	100	0.453
pilA	Acinetobacter baumannii strain A118	43.919	98.667	0.433
pilA	Vibrio parahaemolyticus RIMD 2210633	41.892	98.667	0.413
comP	Acinetobacter baylyi ADP1	41.333	100	0.413
pilA/pilAII	Pseudomonas stutzeri DSM 10701	42.657	95.333	0.407
pilA/pilA1	Eikenella corrodens VA1	39.189	98.667	0.387
pilA	Haemophilus influenzae 86-028NP	36.667	100	0.367
pilA2	Legionella pneumophila str. Paris	36.735	98	0.36
pilA2	Legionella pneumophila strain ERS1305867	36.735	98	0.36