Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABK833_RS25360 Genome accession   NZ_CP157459
Coordinates   5419915..5420367 (-) Length   150 a.a.
NCBI ID   WP_031754077.1    Uniprot ID   P08015
Organism   Pseudomonas aeruginosa PA103     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5414915..5425367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABK833_RS25340 (ABK833_25315) ampD 5415610..5416176 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ABK833_RS25345 (ABK833_25320) - 5416327..5418582 (-) 2256 WP_031754075.1 DUF1631 domain-containing protein -
  ABK833_RS25350 (ABK833_25325) nadC 5418868..5419716 (+) 849 WP_003161760.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ABK833_RS25360 (ABK833_25335) pilA 5419915..5420367 (-) 453 WP_031754077.1 pilin Machinery gene
  ABK833_RS25365 (ABK833_25340) pilB 5420599..5422299 (+) 1701 WP_023094327.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABK833_RS25370 (ABK833_25345) pilC 5422303..5423523 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  ABK833_RS25375 (ABK833_25350) pilD 5423527..5424399 (+) 873 WP_019486071.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ABK833_RS25380 (ABK833_25355) coaE 5424396..5425007 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  ABK833_RS25385 (ABK833_25360) yacG 5425004..5425204 (+) 201 WP_019486072.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15799.09 Da        Isoelectric Point: 6.3229

>NTDB_id=1007024 ABK833_RS25360 WP_031754077.1 5419915..5420367(-) (pilA) [Pseudomonas aeruginosa PA103]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKILIGTTASTADTTYV
GIDEKANKLGTVAVTIKDTGDGTIKFNFATGQSSPKNAGREITLNRTAEGVWTCTSTQEEMFIPKGCNEP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=1007024 ABK833_RS25360 WP_031754077.1 5419915..5420367(-) (pilA) [Pseudomonas aeruginosa PA103]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAGCTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGCGCTTCGGCGCTGGCGACGATCAACCCGTTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTCTGATCGGTACTACAGCTTCTACTGCAGATACCACCTATGTA
GGTATTGATGAGAAGGCAAATAAACTTGGTACCGTAGCTGTAACTATTAAAGACACAGGCGATGGTACTATAAAATTTAA
TTTTGCAACTGGTCAGTCCAGTCCGAAGAATGCGGGCAGGGAAATTACTTTGAATCGTACTGCTGAAGGTGTATGGACTT
GCACCTCTACTCAGGAAGAGATGTTTATTCCTAAGGGTTGTAATGAGCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P08015

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

65.132

100

0.66
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

46.622

98.667

0.46
  pilA Vibrio cholerae strain A1552

46.622

98.667

0.46
  pilA Vibrio cholerae C6706

46.622

98.667

0.46
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.333

100

0.453
  pilA Acinetobacter baumannii strain A118

43.919

98.667

0.433
  pilA Vibrio parahaemolyticus RIMD 2210633

41.892

98.667

0.413
  comP Acinetobacter baylyi ADP1

41.333

100

0.413
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.657

95.333

0.407
  pilA/pilA1 Eikenella corrodens VA1

39.189

98.667

0.387
  pilA Haemophilus influenzae 86-028NP

36.667

100

0.367
  pilA2 Legionella pneumophila str. Paris

36.735

98

0.36
  pilA2 Legionella pneumophila strain ERS1305867

36.735

98

0.36

Multiple sequence alignment