Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ABHF54_RS08650 Genome accession   NZ_CP157069
Coordinates   1832711..1833175 (-) Length   154 a.a.
NCBI ID   WP_347887882.1    Uniprot ID   -
Organism   Nitrosomonas europaea strain CCTCC M 2024693 isolate miaainf     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1827711..1838175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHF54_RS08615 (ABHF54_08610) - 1827910..1828401 (+) 492 WP_323850814.1 VCBS repeat-containing protein -
  ABHF54_RS08620 (ABHF54_08615) - 1828465..1828767 (-) 303 WP_011111882.1 HigA family addiction module antitoxin -
  ABHF54_RS08625 (ABHF54_08620) - 1828780..1829058 (-) 279 WP_276679419.1 type II toxin-antitoxin system RelE/ParE family toxin -
  ABHF54_RS08630 (ABHF54_08625) - 1829186..1829512 (-) 327 WP_323850813.1 type II toxin-antitoxin system PemK/MazF family toxin -
  ABHF54_RS08635 (ABHF54_08630) - 1829509..1829733 (-) 225 WP_041356736.1 antitoxin MazE family protein -
  ABHF54_RS08640 (ABHF54_08635) - 1830272..1831444 (-) 1173 WP_323850812.1 serine hydrolase domain-containing protein -
  ABHF54_RS08645 (ABHF54_08640) - 1831441..1832631 (-) 1191 WP_347887881.1 DUF1295 domain-containing protein -
  ABHF54_RS08650 (ABHF54_08645) pilA/pilAI 1832711..1833175 (-) 465 WP_347887882.1 pilin Machinery gene
  ABHF54_RS08655 (ABHF54_08650) - 1833300..1834025 (-) 726 WP_347887883.1 DUF4145 domain-containing protein -
  ABHF54_RS08660 (ABHF54_08655) - 1834212..1834436 (+) 225 Protein_1692 DUF736 domain-containing protein -
  ABHF54_RS08665 (ABHF54_08660) - 1834539..1835315 (-) 777 WP_347887884.1 hypothetical protein -
  ABHF54_RS08670 (ABHF54_08665) - 1835399..1836256 (-) 858 Protein_1694 IS3 family transposase -
  ABHF54_RS08675 (ABHF54_08670) - 1836374..1836597 (+) 224 Protein_1695 type VI secretion protein -
  ABHF54_RS08680 (ABHF54_08675) - 1836608..1836769 (+) 162 Protein_1696 TrbI/VirB10 family protein -
  ABHF54_RS08685 (ABHF54_08680) - 1836766..1837008 (+) 243 WP_347887885.1 DUF2274 domain-containing protein -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15286.16 Da        Isoelectric Point: 5.7531

>NTDB_id=1003940 ABHF54_RS08650 WP_347887882.1 1832711..1833175(-) (pilA/pilAI) [Nitrosomonas europaea strain CCTCC M 2024693 isolate miaainf]
MQHTQKGFTLIELMIVVAIIGILAAVAIPAYQDYTVKSNAAAALAEITPGKAGFEQAINEGKTPSTTVTDPGYIGIVKVT
SYCSDVDVSSGTSIVCTTAGGNATNFNGKTITLARSADGVWSCNTNLDAKYAPGKCGAAGGGSTGGSTGGSTGG

Nucleotide


Download         Length: 465 bp        

>NTDB_id=1003940 ABHF54_RS08650 WP_347887882.1 1832711..1833175(-) (pilA/pilAI) [Nitrosomonas europaea strain CCTCC M 2024693 isolate miaainf]
ATGCAACATACCCAAAAAGGTTTTACTTTGATTGAATTGATGATCGTGGTCGCGATCATCGGTATTCTGGCGGCGGTGGC
GATTCCGGCTTATCAGGATTACACCGTTAAATCCAATGCCGCTGCTGCTCTGGCTGAAATTACTCCTGGAAAGGCTGGCT
TTGAACAGGCTATCAACGAGGGGAAAACACCCTCCACGACGGTTACAGATCCAGGTTATATCGGCATCGTAAAAGTGACT
AGCTATTGCAGCGACGTAGATGTAAGCAGCGGAACAAGCATTGTATGCACAACAGCAGGAGGAAACGCCACAAATTTCAA
TGGAAAAACGATCACTTTGGCACGCAGTGCTGATGGCGTTTGGTCCTGTAATACTAACTTGGATGCCAAGTATGCTCCAG
GTAAATGCGGCGCTGCAGGGGGTGGTAGCACTGGCGGTAGCACTGGCGGTTCCACAGGTGGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

74.815

87.662

0.656
  pilA/pilAII Pseudomonas stutzeri DSM 10701

62.879

85.714

0.539
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.737

100

0.429
  pilA Vibrio cholerae C6706

41.781

94.805

0.396
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.781

94.805

0.396
  pilA Vibrio cholerae strain A1552

41.781

94.805

0.396
  pilA Ralstonia pseudosolanacearum GMI1000

37.195

100

0.396
  pilA Acinetobacter baumannii strain A118

42.254

92.208

0.39
  pilA Pseudomonas aeruginosa PAK

40

97.403

0.39
  comP Acinetobacter baylyi ADP1

38.667

97.403

0.377
  pilA2 Legionella pneumophila strain ERS1305867

42.963

87.662

0.377
  pilA2 Legionella pneumophila str. Paris

42.963

87.662

0.377

Multiple sequence alignment