Detailed information    

insolico Bioinformatically predicted

Overview

Name   comP   Type   Machinery gene
Locus tag   ABFU48_RS05260 Genome accession   NZ_CP155976
Coordinates   1251646..1252059 (-) Length   137 a.a.
NCBI ID   WP_323530790.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain 0516     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1246646..1257059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU48_RS05240 (ABFU48_05230) coaE 1246736..1247359 (-) 624 WP_319608027.1 dephospho-CoA kinase -
  ABFU48_RS05245 (ABFU48_05235) - 1247373..1248236 (-) 864 WP_011038216.1 A24 family peptidase -
  ABFU48_RS05250 (ABFU48_05240) pilC 1248243..1249505 (-) 1263 WP_323530792.1 type II secretion system F family protein Machinery gene
  ABFU48_RS05255 (ABFU48_05245) - 1249618..1251579 (-) 1962 WP_323530791.1 hypothetical protein -
  ABFU48_RS05260 (ABFU48_05250) comP 1251646..1252059 (-) 414 WP_323530790.1 pilin Machinery gene
  ABFU48_RS05265 (ABFU48_05255) - 1252382..1252762 (+) 381 WP_323530789.1 SMR family transporter -
  ABFU48_RS05270 (ABFU48_05260) - 1252764..1253330 (+) 567 WP_407469972.1 glycosyltransferase -
  ABFU48_RS05275 (ABFU48_05265) - 1253327..1253560 (+) 234 WP_407469973.1 hypothetical protein -
  ABFU48_RS05280 (ABFU48_05270) - 1253822..1254598 (+) 777 WP_407469974.1 glycosyltransferase family 2 protein -
  ABFU48_RS05285 (ABFU48_05275) - 1254591..1255199 (+) 609 WP_323530787.1 methionine biosynthesis protein MetW -
  ABFU48_RS05290 (ABFU48_05280) pilB 1255292..1257025 (+) 1734 WP_323530786.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14060.02 Da        Isoelectric Point: 8.4765

>NTDB_id=1000581 ABFU48_RS05260 WP_323530790.1 1251646..1252059(-) (comP) [Xanthomonas campestris pv. raphani strain 0516]
MKKNAQGFTLIELMIVIAIIAILAAIALPAYSDYTKKAKVSEVVLAASSGRTTISEYVAANNGTCPNTSVLESQSSTYVS
GVSNSGCVITAVSTVSGATGNIVLTGTVNPSTFQVDWTCGGSVQAKYRPGSCQGTKQ

Nucleotide


Download         Length: 414 bp        

>NTDB_id=1000581 ABFU48_RS05260 WP_323530790.1 1251646..1252059(-) (comP) [Xanthomonas campestris pv. raphani strain 0516]
ATGAAGAAGAATGCACAGGGCTTCACCCTGATCGAACTGATGATCGTGATCGCCATCATCGCCATCCTGGCCGCCATTGC
GCTGCCGGCCTATTCGGACTACACCAAGAAGGCCAAGGTCTCGGAAGTCGTGCTGGCCGCTTCGTCGGGCCGCACCACCA
TTTCGGAGTATGTCGCCGCCAATAACGGCACGTGCCCTAACACCAGCGTGCTAGAGAGCCAGTCGTCGACCTACGTGAGC
GGCGTCAGCAACAGCGGTTGCGTGATCACCGCCGTCAGCACTGTCTCCGGCGCAACCGGTAACATCGTCCTGACCGGCAC
GGTGAATCCCTCCACCTTCCAAGTCGACTGGACCTGCGGCGGCAGCGTGCAGGCTAAGTATCGTCCGGGTTCCTGCCAGG
GCACCAAGCAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-28)

Pilin (bacterial filament) (Pilin)

(37-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Acinetobacter baylyi ADP1

44.966

100

0.489
  pilA2 Legionella pneumophila strain ERS1305867

43.165

100

0.438
  pilA2 Legionella pneumophila str. Paris

44.361

97.08

0.431
  pilA/pilA1 Eikenella corrodens VA1

35.948

100

0.401
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.913

100

0.401
  pilE Neisseria gonorrhoeae MS11

42.969

93.431

0.401
  pilE Neisseria gonorrhoeae strain FA1090

43.307

92.701

0.401
  pilA Acinetobacter baumannii strain A118

37.762

100

0.394
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.241

100

0.394
  pilA Ralstonia pseudosolanacearum GMI1000

33.125

100

0.387

Multiple sequence alignment