Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU48_RS05290 Genome accession   NZ_CP155976
Coordinates   1255292..1257025 (+) Length   577 a.a.
NCBI ID   WP_323530786.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain 0516     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1250292..1262025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU48_RS05260 (ABFU48_05250) comP 1251646..1252059 (-) 414 WP_323530790.1 pilin Machinery gene
  ABFU48_RS05265 (ABFU48_05255) - 1252382..1252762 (+) 381 WP_323530789.1 SMR family transporter -
  ABFU48_RS05270 (ABFU48_05260) - 1252764..1253330 (+) 567 WP_407469972.1 glycosyltransferase -
  ABFU48_RS05275 (ABFU48_05265) - 1253327..1253560 (+) 234 WP_407469973.1 hypothetical protein -
  ABFU48_RS05280 (ABFU48_05270) - 1253822..1254598 (+) 777 WP_407469974.1 glycosyltransferase family 2 protein -
  ABFU48_RS05285 (ABFU48_05275) - 1254591..1255199 (+) 609 WP_323530787.1 methionine biosynthesis protein MetW -
  ABFU48_RS05290 (ABFU48_05280) pilB 1255292..1257025 (+) 1734 WP_323530786.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU48_RS05295 (ABFU48_05285) pilR 1257439..1258833 (-) 1395 WP_323530785.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU48_RS05300 (ABFU48_05290) - 1259043..1260653 (-) 1611 WP_070690016.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62703.73 Da        Isoelectric Point: 5.6021

>NTDB_id=1000582 ABFU48_RS05290 WP_323530786.1 1255292..1257025(+) (pilB) [Xanthomonas campestris pv. raphani strain 0516]
MNTAVTTNLVGITGIARRLVQDGAIDESAARGAMDQAAAAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
NDDDDMGDLDVSSGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1000582 ABFU48_RS05290 WP_323530786.1 1255292..1257025(+) (pilB) [Xanthomonas campestris pv. raphani strain 0516]
ATGAACACCGCTGTTACGACGAATCTAGTCGGTATCACGGGAATCGCTCGTCGCCTGGTGCAGGACGGTGCGATTGACGA
ATCCGCTGCTCGCGGCGCCATGGACCAGGCTGCTGCCGCCAAGGTCCCGCTTCCTCAGTGGTTTGCCGAAAAAAAACTGG
TATCGGCCGCGCAGCTTGCTGCCGCCAACGCCGTTGAGTTCGGGATGCCGCTGATGGATGTGTCGGCGTTCGACGCCAAT
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTTGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTTGACGATATCAAGTTCCATACGAACCTGGTGGTCGAGCCTA
TCCTGGTCGACGAGGACCAGATACGGCGCACACTGGAGCAGTGGCAGGCCAGCAATGCGTCGCTTGGCTCGTCGTTGGGG
AACGACGATGACGATATGGGGGACCTTGACGTGTCGTCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAAGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCACTTCGAGCCCTACGAAGACGACTATCGGGTGCGCTTGCGCATCGACGGCCTGCTGAAGAATGTGGCCAAGGCACCG
GTGAAGTTGAGCCAGCGCATCGCTGCACGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCAGCCAAGCTGGGTATCGAGAAACTGGGCTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAACCTTATGGCATGGTGCTGGTGACCGGTCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTACACGGCGTTGGGCATCCTCAACGATGAGACGCGCAACATTTCGACCGCCGAGGATCCGGTCGAAATCCGCCTGCCCG
GCGTCAACCAGGTCCAGCAGAACAATAAGCGCGGCATGACCTTCGCCGCGGCCTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACGGGCCACATGGT
GCTGTCGACACTGCACACCAACGATGCGCCCCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATTA
CCTCATCGGTGACCCTGGTCATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
ATGCACGGAAGGCTACAAAGGCCGTACCGGCATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTAATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAGATCAATCGTGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.458

98.44

0.546
  pilB Acinetobacter baumannii D1279779

55.282

98.44

0.544
  pilB Legionella pneumophila strain ERS1305867

52.083

99.827

0.52
  pilB Vibrio cholerae strain A1552

48.87

99.653

0.487
  pilF Neisseria gonorrhoeae MS11

48.592

98.44

0.478
  pilB Vibrio campbellii strain DS40M4

45.87

98.614

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

45.648

97.574

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

35.246

100

0.373

Multiple sequence alignment