Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ABFU48_RS05250 Genome accession   NZ_CP155976
Coordinates   1248243..1249505 (-) Length   420 a.a.
NCBI ID   WP_323530792.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain 0516     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1243243..1254505
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU48_RS05220 (ABFU48_05210) - 1243473..1244807 (+) 1335 WP_012437598.1 HAMP domain-containing sensor histidine kinase -
  ABFU48_RS05225 (ABFU48_05215) - 1244950..1245441 (-) 492 WP_323530794.1 GNAT family N-acetyltransferase -
  ABFU48_RS05230 (ABFU48_05220) - 1245438..1245728 (-) 291 WP_014508712.1 DUF1778 domain-containing protein -
  ABFU48_RS05235 (ABFU48_05225) - 1245803..1246204 (-) 402 WP_323530793.1 SymE family type I addiction module toxin -
  ABFU48_RS05240 (ABFU48_05230) coaE 1246736..1247359 (-) 624 WP_319608027.1 dephospho-CoA kinase -
  ABFU48_RS05245 (ABFU48_05235) - 1247373..1248236 (-) 864 WP_011038216.1 A24 family peptidase -
  ABFU48_RS05250 (ABFU48_05240) pilC 1248243..1249505 (-) 1263 WP_323530792.1 type II secretion system F family protein Machinery gene
  ABFU48_RS05255 (ABFU48_05245) - 1249618..1251579 (-) 1962 WP_323530791.1 hypothetical protein -
  ABFU48_RS05260 (ABFU48_05250) comP 1251646..1252059 (-) 414 WP_323530790.1 pilin Machinery gene
  ABFU48_RS05265 (ABFU48_05255) - 1252382..1252762 (+) 381 WP_323530789.1 SMR family transporter -
  ABFU48_RS05270 (ABFU48_05260) - 1252764..1253330 (+) 567 WP_407469972.1 glycosyltransferase -
  ABFU48_RS05275 (ABFU48_05265) - 1253327..1253560 (+) 234 WP_407469973.1 hypothetical protein -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45889.43 Da        Isoelectric Point: 10.3578

>NTDB_id=1000580 ABFU48_RS05250 WP_323530792.1 1248243..1249505(-) (pilC) [Xanthomonas campestris pv. raphani strain 0516]
MSALGSTVKKGAKPANRVNAMTSFVWEGTDKRGVKMKGDELARNANMLRAELRRRGINPTVVKTKPKPLFGAAGKPIKPK
DIAFFSRQIATMMKSGVPMVGTLEIIGEGSKNPRMRKMVGEIRTDIEGGSSLNEAISRHPVQFDELYRNLVRAGESAGVL
DTVLDTVATYKENIEALKGKIKKALFYPAMVMAVALIVSSILLIWVVPQFETVFSSFGAELPAFTQMIVNLSRFMVSWWF
PILLVIIGSAVGLIMAYKRSPKMQHLFDRLILKVPVIGKIMHDSSIARFARTTAVTFKAGVPLVEALGIVAGATGNKVYE
EAVLRMRDDVSVGYPVNLSMKQVNLFPHMVIQMTAIGEEAGALDAMLFKVAEYFEQEVNNAVDALSSLLEPMIMIFIGTI
VGGMVIGMYLPIFKLGAVVG

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=1000580 ABFU48_RS05250 WP_323530792.1 1248243..1249505(-) (pilC) [Xanthomonas campestris pv. raphani strain 0516]
ATGTCAGCACTGGGCAGCACTGTGAAAAAAGGGGCTAAACCGGCCAATCGCGTTAACGCGATGACGTCGTTCGTCTGGGA
GGGGACAGACAAGCGCGGCGTCAAAATGAAGGGAGACGAGTTGGCTCGCAATGCGAACATGCTGCGCGCCGAACTTCGCC
GTAGGGGGATCAATCCCACTGTCGTCAAGACAAAGCCCAAGCCGCTGTTTGGCGCAGCCGGCAAGCCGATCAAACCGAAG
GACATCGCATTTTTCAGTCGCCAGATTGCGACCATGATGAAGTCAGGCGTTCCCATGGTAGGCACCCTGGAAATCATAGG
CGAGGGCTCCAAAAATCCCCGCATGCGAAAGATGGTTGGCGAAATCCGTACCGACATCGAAGGCGGATCATCGCTCAATG
AAGCGATCAGCAGACATCCAGTGCAGTTTGACGAGCTCTATCGCAACTTGGTCCGGGCAGGCGAAAGCGCCGGCGTCCTG
GACACGGTGCTGGATACTGTGGCCACCTACAAGGAAAATATCGAAGCACTGAAGGGCAAGATCAAGAAAGCCCTGTTTTA
TCCTGCGATGGTGATGGCCGTAGCGTTGATCGTCAGTTCCATATTGCTGATCTGGGTGGTACCGCAGTTCGAGACGGTAT
TCTCCAGCTTCGGTGCTGAATTGCCTGCATTCACCCAGATGATCGTGAATCTTTCGCGATTCATGGTTTCATGGTGGTTC
CCGATCCTGCTGGTGATCATTGGGTCGGCGGTGGGGCTGATCATGGCCTACAAACGCTCGCCCAAGATGCAACACCTGTT
TGATCGTCTGATTCTGAAAGTGCCAGTCATTGGCAAGATCATGCATGACAGTTCGATTGCCCGATTCGCACGGACGACCG
CCGTCACTTTCAAAGCAGGCGTTCCATTGGTCGAAGCCCTGGGAATCGTCGCGGGCGCGACAGGTAACAAGGTCTACGAG
GAAGCAGTTCTGCGGATGCGCGACGACGTCTCCGTAGGCTACCCAGTCAATCTGTCGATGAAGCAGGTCAACCTGTTTCC
TCACATGGTGATTCAGATGACGGCGATCGGTGAAGAGGCCGGCGCACTGGATGCCATGCTGTTCAAGGTTGCAGAATACT
TCGAGCAGGAAGTCAACAATGCCGTCGATGCACTGAGCAGCCTGCTTGAGCCTATGATCATGATCTTCATCGGCACCATC
GTCGGCGGCATGGTCATCGGCATGTATCTTCCGATCTTCAAACTCGGCGCAGTGGTTGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(289-411)

Type II secretion system (T2SS), protein F (T2SSF)

(85-208)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.04

94.286

0.51
  pilC Legionella pneumophila strain ERS1305867

51.128

95

0.486
  pilC Acinetobacter baylyi ADP1

50.123

96.905

0.486
  pilC Acinetobacter baumannii D1279779

48.395

96.429

0.467
  pilG Neisseria meningitidis 44/76-A

43.176

95.952

0.414
  pilG Neisseria gonorrhoeae MS11

42.643

95.476

0.407
  pilC Vibrio cholerae strain A1552

39.163

96.667

0.379
  pilC Vibrio campbellii strain DS40M4

39

95.238

0.371
  pilC Thermus thermophilus HB27

38.366

96.19

0.369

Multiple sequence alignment