Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ABFU38_RS05470 Genome accession   NZ_CP155967
Coordinates   1299958..1300410 (+) Length   150 a.a.
NCBI ID   WP_407469261.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain BXC#141     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1294958..1305410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU38_RS05445 (ABFU38_05440) - 1294978..1296312 (+) 1335 WP_019237347.1 HAMP domain-containing sensor histidine kinase -
  ABFU38_RS05450 - 1296411..1296578 (-) 168 Protein_1060 SymE family type I addiction module toxin -
  ABFU38_RS05455 (ABFU38_05450) coaE 1296860..1297483 (-) 624 WP_225042504.1 dephospho-CoA kinase -
  ABFU38_RS05460 (ABFU38_05455) - 1297497..1298360 (-) 864 WP_407469259.1 prepilin peptidase -
  ABFU38_RS05465 (ABFU38_05460) pilC 1298367..1299620 (-) 1254 WP_407469260.1 type II secretion system F family protein Machinery gene
  ABFU38_RS05470 (ABFU38_05465) pilA2 1299958..1300410 (+) 453 WP_407469261.1 pilin Machinery gene
  ABFU38_RS05475 (ABFU38_05470) - 1300481..1301089 (+) 609 WP_407469262.1 methyltransferase family protein -
  ABFU38_RS05480 (ABFU38_05475) - 1301093..1302253 (+) 1161 WP_407469263.1 hypothetical protein -
  ABFU38_RS05485 (ABFU38_05480) pilB 1302307..1304040 (+) 1734 WP_407469264.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU38_RS05490 (ABFU38_05485) - 1304223..1305014 (-) 792 WP_407462317.1 toxin -
  ABFU38_RS05495 (ABFU38_05490) - 1304959..1305315 (-) 357 WP_139328384.1 hypothetical protein -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15434.88 Da        Isoelectric Point: 8.4723

>NTDB_id=1000424 ABFU38_RS05470 WP_407469261.1 1299958..1300410(+) (pilA2) [Xanthomonas campestris pv. raphani strain BXC#141]
MKKQQGFTLIELMIVIAIIAILAAIALPAYQDYTIRAKVSETMIAMSAAKLAVSETAQSQGILATSVLDNTAAGYSGQLT
DKVASVVIANGIITGTSKNTGAKVEPILKLTPSQTDKDAPITWACSYSAGESKHVPASCRTAAPTPAPAG

Nucleotide


Download         Length: 453 bp        

>NTDB_id=1000424 ABFU38_RS05470 WP_407469261.1 1299958..1300410(+) (pilA2) [Xanthomonas campestris pv. raphani strain BXC#141]
ATGAAGAAGCAGCAAGGTTTCACACTTATCGAGTTGATGATCGTTATTGCGATCATTGCAATTCTGGCCGCAATTGCTTT
GCCTGCGTACCAGGATTACACGATTCGCGCCAAAGTAAGCGAAACGATGATCGCAATGTCTGCTGCAAAGCTGGCCGTCT
CGGAAACTGCGCAGTCTCAAGGCATTCTTGCAACGTCAGTTCTTGATAATACTGCCGCGGGATATTCTGGTCAGCTCACC
GATAAGGTTGCTAGCGTAGTGATCGCGAATGGTATTATTACTGGAACGTCGAAGAACACCGGTGCTAAAGTTGAGCCGAT
TTTGAAGCTGACTCCTTCTCAGACAGATAAGGATGCTCCGATCACTTGGGCTTGCAGCTATAGTGCTGGCGAATCGAAGC
ACGTGCCTGCGAGCTGCCGTACCGCTGCTCCGACTCCTGCTCCGGCTGGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.064

94

0.48
  pilA2 Legionella pneumophila strain ERS1305867

50.355

94

0.473
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.75

100

0.467
  comP Acinetobacter baylyi ADP1

42.667

100

0.427
  pilE Neisseria gonorrhoeae strain FA1090

40.764

100

0.427
  pilA Ralstonia pseudosolanacearum GMI1000

37.278

100

0.42
  pilE Neisseria gonorrhoeae MS11

37.888

100

0.407
  pilA Haemophilus influenzae Rd KW20

39.86

95.333

0.38
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.716

94

0.373
  pilA Haemophilus influenzae 86-028NP

38.298

94

0.36
  pilA/pilA1 Eikenella corrodens VA1

36

100

0.36

Multiple sequence alignment