Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFU38_RS05485 Genome accession   NZ_CP155967
Coordinates   1302307..1304040 (+) Length   577 a.a.
NCBI ID   WP_407469264.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain BXC#141     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1297307..1309040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU38_RS05460 (ABFU38_05455) - 1297497..1298360 (-) 864 WP_407469259.1 prepilin peptidase -
  ABFU38_RS05465 (ABFU38_05460) pilC 1298367..1299620 (-) 1254 WP_407469260.1 type II secretion system F family protein Machinery gene
  ABFU38_RS05470 (ABFU38_05465) pilA2 1299958..1300410 (+) 453 WP_407469261.1 pilin Machinery gene
  ABFU38_RS05475 (ABFU38_05470) - 1300481..1301089 (+) 609 WP_407469262.1 methyltransferase family protein -
  ABFU38_RS05480 (ABFU38_05475) - 1301093..1302253 (+) 1161 WP_407469263.1 hypothetical protein -
  ABFU38_RS05485 (ABFU38_05480) pilB 1302307..1304040 (+) 1734 WP_407469264.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU38_RS05490 (ABFU38_05485) - 1304223..1305014 (-) 792 WP_407462317.1 toxin -
  ABFU38_RS05495 (ABFU38_05490) - 1304959..1305315 (-) 357 WP_139328384.1 hypothetical protein -
  ABFU38_RS05500 (ABFU38_05495) pilR 1305513..1306907 (-) 1395 WP_070690014.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU38_RS05505 (ABFU38_05500) - 1307116..1308726 (-) 1611 WP_407460383.1 sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62934.17 Da        Isoelectric Point: 5.7626

>NTDB_id=1000425 ABFU38_RS05485 WP_407469264.1 1302307..1304040(+) (pilB) [Xanthomonas campestris pv. raphani strain BXC#141]
MSTVINPNLVGITGIARRLVQDGALDETIARSAMDHAAAAKVPLPQWFAERKLVTASQLAAANAIEFGMPLLDVSVFDAN
QNAIKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSSNAALGSSMG
DDDEGMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1000425 ABFU38_RS05485 WP_407469264.1 1302307..1304040(+) (pilB) [Xanthomonas campestris pv. raphani strain BXC#141]
ATGAGTACCGTAATCAATCCCAATCTGGTAGGCATTACCGGTATCGCGCGGCGCCTTGTGCAGGATGGTGCATTAGATGA
AACGATCGCTCGATCCGCTATGGATCACGCTGCTGCAGCTAAGGTTCCTCTGCCACAGTGGTTTGCAGAGAGGAAACTGG
TAACCGCTTCTCAGTTAGCGGCTGCTAATGCAATCGAGTTTGGAATGCCGCTGCTCGACGTTTCGGTCTTCGACGCCAAC
CAGAACGCAATCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTGGACGACATCAAGTTTCATACGAACCTGGTGGTCGAGCCGA
TCCTGGTTGATGAGGACCAGATTCGTCGCACACTGGAGCAGTGGCAGTCCAGCAATGCGGCGCTTGGTTCGTCGATGGGT
GACGACGACGAGGGGATGGGGGATCTGGACGTCTCCACTGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGTGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGTTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGATTTTCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGCGCGGCCAAGCTCGGTATCGAGAAGCTGGGTTACGAGGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACCGTGTCGCT
GTATACCGCGCTCGGGATTCTCAACGACGAGACCCGCAATATTTCCACGGCCGAGGATCCGGTCGAAATCCGCCTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCTGCGGCGCTGCGCTCGTTCCTGCGTCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACTGGCCACATGGT
GCTGTCGACACTGCACACCAACGATGCGCCGCAAACCATTGCGCGTCTGATGAACATGGGTATCGCGCCGTACAACATCA
CCTCCTCGGTGACCCTGGTCATCGCACAGCGCTTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCG
GAACATGCGCTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTTGCGAACATCGAGCTCTATGAAGCGGTCGGTTGCGATGA
ATGCACCGAAGGCTACAAAGGCCGTACCGGCATCTATCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTGATGAAG
GCTTCGCACGGCGTGACCAGCCTGGCCGAAATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.81

98.44

0.549
  pilB Acinetobacter baumannii D1279779

55.81

98.44

0.549
  pilB Legionella pneumophila strain ERS1305867

52.289

98.44

0.515
  pilB Vibrio cholerae strain A1552

48.785

99.827

0.487
  pilF Neisseria gonorrhoeae MS11

48.944

98.44

0.482
  pilB Vibrio campbellii strain DS40M4

45.694

98.614

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

45.826

97.574

0.447

Multiple sequence alignment