Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type I Classification (family/domain) ldrD-rdlD/-
Location 1271280..1271500 Replicon chromosome
Accession NC_007779
Organism Escherichia coli str. K-12 substr. W3110
T1TAdb ID TA06175

Toxin (Protein)


Gene name ldrD Uniprot ID J7R083
Locus tag Y75_p1189 Protein ID WP_000170963.1
Coordinates 1271280..1271387 (-) Length 36 a.a.

Antitoxin (RNA)


Gene name rdlD
Locus tag Y75_s0009
Coordinates 1271434..1271500 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y75_RS06335 (Y75_p1183) 1266589..1267671 + 1083 WP_000804726.1 peptide chain release factor 1 -
Y75_RS06340 (Y75_p1184) 1267671..1268504 + 834 WP_000456467.1 peptide chain release factor N(5)-glutamine methyltransferase -
Y75_RS06345 (Y75_p1185) 1268501..1268893 + 393 WP_000200374.1 invasion regulator SirB2 -
Y75_RS06350 (Y75_p1186) 1268897..1269706 + 810 WP_001257044.1 invasion regulator SirB1 -
Y75_RS06355 (Y75_p1187) 1269742..1270596 + 855 WP_000811065.1 3-deoxy-8-phosphooctulonate synthase -
Y75_RS06360 (Y75_p1188) 1270745..1270852 - 108 WP_000170955.1 small toxic polypeptide LdrA/LdrC -
Y75_RS06365 (Y75_p1189) 1271280..1271387 - 108 WP_000170963.1 small toxic polypeptide LdrB Toxin
- 1271434..1271500 + 67 - - Antitoxin
Y75_RS06370 (Y75_p1190) 1271815..1271922 - 108 WP_000170955.1 small toxic polypeptide LdrA/LdrC -
Y75_RS06375 (Y75_p1191) 1272326..1273426 - 1101 WP_000063607.1 sodium-potassium/proton antiporter ChaA -
Y75_RS06380 (Y75_p1192) 1273696..1273926 + 231 WP_001146444.1 putative cation transport regulator ChaB -
Y75_RS06385 (Y75_p1193) 1274084..1274779 + 696 WP_001336325.1 glutathione-specific gamma-glutamylcyclotransferase -
Y75_RS06390 (Y75_p1194) 1274823..1275176 - 354 WP_001169669.1 DsrE/F sulfur relay family protein YchN -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-35)


Sequences


Toxin        


Download         Length: 36 a.a.        Molecular weight: 3971.74 Da        Isoelectric Point: 11.4779

>T6342 WP_000170963.1 NC_007779:c1271387-1271280 [Escherichia coli str. K-12 substr. W3110]
MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK

Download         Length: 108 bp

>T6342 NC_007779:c1271387-1271280 [Escherichia coli str. K-12 substr. W3110]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 67 bp

>AT6342 NC_007779:1271434-1271500 [Escherichia coli str. K-12 substr. W3110]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T6338 Escherichia coli str. K-12 substr. MDS42

100

100

1

T6334 Escherichia coli str. K-12 substr. DH10B

100

100

1

T6330 Escherichia coli str. K-12 substr. MG1655

100

100

1

T6326 Escherichia coli str. K-12 substr. MC4100

100

100

1

T6346 Escherichia coli K-12 strain ER3413

100

100

1

T6343 Escherichia coli str. K-12 substr. W3110

94.286

100

0.943

T6341 Escherichia coli str. K-12 substr. W3110

94.286

100

0.943

T6331 Escherichia coli str. K-12 substr. MG1655

94.286

100

0.943

T6329 Escherichia coli str. K-12 substr. MG1655

94.286

100

0.943

T6347 Escherichia coli K-12 strain ER3413

94.286

100

0.943

T6327 Escherichia coli str. K-12 substr. MC4100

94.286

100

0.943

T6325 Escherichia coli str. K-12 substr. MC4100

94.286

100

0.943

T6345 Escherichia coli K-12 strain ER3413

94.286

100

0.943

T6339 Escherichia coli str. K-12 substr. MDS42

94.286

100

0.943

T6337 Escherichia coli str. K-12 substr. MDS42

94.286

100

0.943

T6335 Escherichia coli str. K-12 substr. DH10B

94.286

100

0.943

T6333 Escherichia coli str. K-12 substr. DH10B

94.286

100

0.943

T6332 Escherichia coli str. K-12 substr. MG1655

57.143

100

0.571

T6348 Escherichia coli K-12 strain ER3413

57.143

100

0.571

T6336 Escherichia coli str. K-12 substr. DH10B

57.143

100

0.571

T6344 Escherichia coli str. K-12 substr. W3110

57.143

100

0.571

T6340 Escherichia coli str. K-12 substr. MDS42

57.143

100

0.571

T6328 Escherichia coli str. K-12 substr. MC4100

57.143

100

0.571


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Download structure file

References


(1) Mitsuoki Kawano et al. (2002) Molecular characterization of long direct repeat (LDR) sequences expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide and a cis-encoded small antisense RNA in Escherichia coli. Molecular Microbiology 45(2):333-49. [PubMed:12123448] experimental

(2) Elizabeth M Fozo et al. (2010) Abundance of type I toxin-antitoxin systems in bacteria: searches for new candidates and discovery of novel families. Nucleic Acids Research 38(11):3743-59. [PubMed:20156992] experimental


experimental experimental literature