Detailed information of TA system TA006339

Experimentally validated

Overview

TA module

• Type: I
• Classification (family/domain): ldrD-rdlD/-
• Location: 1075666..1075888
• Replicon: chromosome
• Accession: NC_020518
• Organism: Escherichia coli str. K-12 substr. MDS42

Toxin (Protein)

• Gene name: ldrD
• Uniprot ID: D3H2K1
• Locus tag: ECMDS42_1007
• Protein ID: WP_000170955.1
• Coordinates: 1075666..1075773 (-)
• Length: 36 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	ECMDS42_1008
Coordinates	1075821..1075888 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Sequences

Toxin

Protein

• Length: 36 a.a.
• Molecular weight: 4013.82 Da
• Isoelectric Point: 11.4779

>T6339 WP_000170955.1 NC_020518:c1075773-1075666 [Escherichia coli str. K-12 substr. MDS42]
MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK

Nucleotide

• Length: 108 bp

>T6339 NC_020518:c1075773-1075666 [Escherichia coli str. K-12 substr. MDS42]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 68 bp

>AT6339 NC_020518:1075821-1075888 [Escherichia coli str. K-12 substr. MDS42]
GTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCT

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6329	Escherichia coli str. K-12 substr. MG1655	100	100	1
T6331	Escherichia coli str. K-12 substr. MG1655	100	100	1
T6341	Escherichia coli str. K-12 substr. W3110	100	100	1
T6343	Escherichia coli str. K-12 substr. W3110	100	100	1
T6333	Escherichia coli str. K-12 substr. DH10B	100	100	1
T6335	Escherichia coli str. K-12 substr. DH10B	100	100	1
T6337	Escherichia coli str. K-12 substr. MDS42	100	100	1
T6327	Escherichia coli str. K-12 substr. MC4100	100	100	1
T6325	Escherichia coli str. K-12 substr. MC4100	100	100	1
T6347	Escherichia coli K-12 strain ER3413	100	100	1
T6345	Escherichia coli K-12 strain ER3413	100	100	1
T6330	Escherichia coli str. K-12 substr. MG1655	94.286	100	0.943
T6342	Escherichia coli str. K-12 substr. W3110	94.286	100	0.943
T6338	Escherichia coli str. K-12 substr. MDS42	94.286	100	0.943
T6326	Escherichia coli str. K-12 substr. MC4100	94.286	100	0.943
T6346	Escherichia coli K-12 strain ER3413	94.286	100	0.943
T6334	Escherichia coli str. K-12 substr. DH10B	94.286	100	0.943
T6336	Escherichia coli str. K-12 substr. DH10B	57.143	100	0.571
T6348	Escherichia coli K-12 strain ER3413	57.143	100	0.571
T6332	Escherichia coli str. K-12 substr. MG1655	57.143	100	0.571
T6344	Escherichia coli str. K-12 substr. W3110	57.143	100	0.571
T6328	Escherichia coli str. K-12 substr. MC4100	57.143	100	0.571
T6340	Escherichia coli str. K-12 substr. MDS42	57.143	100	0.571

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: A0A829J2A9

Antitoxin

References

(1) Mitsuoki Kawano et al. (2002) Molecular characterization of long direct repeat (LDR) sequences expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide and a cis-encoded small antisense RNA in Escherichia coli. Molecular Microbiology 45(2):333-49. [PubMed:12123448]


 experimental literature