Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type I Classification (family/domain) ldrD-rdlD/-
Location 1309702..1309924 Replicon chromosome
Accession NC_010473
Organism Escherichia coli str. K-12 substr. DH10B
T1TAdb ID TA06259

Toxin (Protein)


Gene name ldrD Uniprot ID D3H2K1
Locus tag ECDH10B_1273 Protein ID WP_000170955.1
Coordinates 1309702..1309809 (-) Length 36 a.a.

Antitoxin (RNA)


Gene name rdlD
Locus tag ECDH10B_1274
Coordinates 1309865..1309921 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH10B_RS06520 (ECDH10B_1265) 1305558..1306391 + 834 WP_000456467.1 peptide chain release factor N(5)-glutamine methyltransferase -
ECDH10B_RS06525 (ECDH10B_1266) 1306388..1306780 + 393 WP_000200374.1 invasion regulator SirB2 -
ECDH10B_RS06530 (ECDH10B_1267) 1306784..1307593 + 810 WP_001257044.1 invasion regulator SirB1 -
ECDH10B_RS06535 (ECDH10B_1268) 1307629..1308483 + 855 WP_000811065.1 3-deoxy-8-phosphooctulonate synthase -
ECDH10B_RS06540 (ECDH10B_1269) 1308632..1308739 - 108 WP_000170955.1 small toxic polypeptide LdrA/LdrC -
ECDH10B_RS06545 (ECDH10B_1271) 1309167..1309274 - 108 WP_000170963.1 small toxic polypeptide LdrB -
ECDH10B_RS06550 (ECDH10B_1273) 1309702..1309809 - 108 WP_000170955.1 small toxic polypeptide LdrA/LdrC Toxin
- 1309865..1309921 + 57 - - Antitoxin
ECDH10B_RS06555 (ECDH10B_1275) 1310213..1311313 - 1101 WP_000063607.1 sodium-potassium/proton antiporter ChaA -
ECDH10B_RS06560 (ECDH10B_1276) 1311583..1311813 + 231 WP_001146444.1 putative cation transport regulator ChaB -
ECDH10B_RS06565 (ECDH10B_1277) 1311971..1312666 + 696 WP_001336325.1 glutathione-specific gamma-glutamylcyclotransferase -
ECDH10B_RS06570 (ECDH10B_1278) 1312710..1313063 - 354 WP_001169669.1 DsrE/F sulfur relay family protein YchN -
ECDH10B_RS06575 (ECDH10B_1279) 1313248..1314642 + 1395 WP_000086217.1 inverse autotransporter invasin YchO -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-35)


Sequences


Toxin        


Download         Length: 36 a.a.        Molecular weight: 4013.82 Da        Isoelectric Point: 11.4779

>T6335 WP_000170955.1 NC_010473:c1309809-1309702 [Escherichia coli str. K-12 substr. DH10B]
MTLAQFAMIFWHDLAAPILAGIITAAIVSWWRNRK

Download         Length: 108 bp

>T6335 NC_010473:c1309809-1309702 [Escherichia coli str. K-12 substr. DH10B]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 57 bp

>AT6335 NC_010473:1309865-1309921 [Escherichia coli str. K-12 substr. DH10B]
TCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829J2A9


Antitoxin

Download structure file

References


(1) Mitsuoki Kawano et al. (2002) Molecular characterization of long direct repeat (LDR) sequences expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide and a cis-encoded small antisense RNA in Escherichia coli. Molecular Microbiology 45(2):333-49. [PubMed:12123448] experimental

(2) Elizabeth M Fozo et al. (2010) Abundance of type I toxin-antitoxin systems in bacteria: searches for new candidates and discovery of novel families. Nucleic Acids Research 38(11):3743-59. [PubMed:20156992] experimental


experimental experimental literature