Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ABC804_RS01390 Genome accession   NZ_CP155536
Coordinates   261521..262462 (+) Length   313 a.a.
NCBI ID   WP_000249552.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain M264-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 256521..267462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABC804_RS01370 (ABC804_01370) - 256587..258404 (+) 1818 WP_001220922.1 acyltransferase family protein -
  ABC804_RS01375 (ABC804_01375) nagA 258557..259708 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  ABC804_RS01380 (ABC804_01380) - 259871..260929 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  ABC804_RS01385 (ABC804_01385) - 261080..261445 (+) 366 WP_000286415.1 DUF1033 family protein -
  ABC804_RS01390 (ABC804_01390) comGA/cglA/cilD 261521..262462 (+) 942 WP_000249552.1 competence type IV pilus ATPase ComGA Machinery gene
  ABC804_RS01395 (ABC804_01395) comGB/cglB 262410..263426 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABC804_RS01400 (ABC804_01400) comGC/cglC 263428..263754 (+) 327 WP_000738636.1 comG operon protein ComGC Machinery gene
  ABC804_RS01405 (ABC804_01405) comGD/cglD 263747..264151 (+) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABC804_RS01410 (ABC804_01410) comGE/cglE 264114..264416 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABC804_RS01415 (ABC804_01415) comGF/cglF 264379..264840 (+) 462 WP_000250542.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABC804_RS01420 (ABC804_01420) comGG/cglG 264818..265231 (+) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABC804_RS01425 (ABC804_01425) - 265368..265962 (+) 595 Protein_258 methyltransferase domain-containing protein -
  ABC804_RS01430 (ABC804_01430) comYH 266023..266976 (+) 954 WP_000345099.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35587.57 Da        Isoelectric Point: 6.0083

>NTDB_id=997395 ABC804_RS01390 WP_000249552.1 261521..262462(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain M264-3]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=997395 ABC804_RS01390 WP_000249552.1 261521..262462(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain M264-3]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGCTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGTCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACATCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.403

100

0.984
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment