Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ABC804_RS01395 Genome accession   NZ_CP155536
Coordinates   262410..263426 (+) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain M264-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 257410..268426
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABC804_RS01375 (ABC804_01375) nagA 258557..259708 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  ABC804_RS01380 (ABC804_01380) - 259871..260929 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  ABC804_RS01385 (ABC804_01385) - 261080..261445 (+) 366 WP_000286415.1 DUF1033 family protein -
  ABC804_RS01390 (ABC804_01390) comGA/cglA/cilD 261521..262462 (+) 942 WP_000249552.1 competence type IV pilus ATPase ComGA Machinery gene
  ABC804_RS01395 (ABC804_01395) comGB/cglB 262410..263426 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABC804_RS01400 (ABC804_01400) comGC/cglC 263428..263754 (+) 327 WP_000738636.1 comG operon protein ComGC Machinery gene
  ABC804_RS01405 (ABC804_01405) comGD/cglD 263747..264151 (+) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABC804_RS01410 (ABC804_01410) comGE/cglE 264114..264416 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABC804_RS01415 (ABC804_01415) comGF/cglF 264379..264840 (+) 462 WP_000250542.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABC804_RS01420 (ABC804_01420) comGG/cglG 264818..265231 (+) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABC804_RS01425 (ABC804_01425) - 265368..265962 (+) 595 Protein_258 methyltransferase domain-containing protein -
  ABC804_RS01430 (ABC804_01430) comYH 266023..266976 (+) 954 WP_000345099.1 class I SAM-dependent methyltransferase Machinery gene
  ABC804_RS01435 (ABC804_01435) - 267027..268217 (+) 1191 WP_000167765.1 acetate kinase -
  ABC804_RS01440 (ABC804_01440) - 268218..268349 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=997396 ABC804_RS01395 WP_074017570.1 262410..263426(+) (comGB/cglB) [Streptococcus pneumoniae strain M264-3]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=997396 ABC804_RS01395 WP_074017570.1 262410..263426(+) (comGB/cglB) [Streptococcus pneumoniae strain M264-3]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment