Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MasN3_RS05865 Genome accession   NZ_AP026966
Coordinates   1270201..1271337 (-) Length   378 a.a.
NCBI ID   WP_281912976.1    Uniprot ID   -
Organism   Massilia varians strain N-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1265201..1276337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MasN3_RS05850 (MasN3_11460) - 1266639..1267325 (+) 687 WP_281912972.1 pentapeptide repeat-containing protein -
  MasN3_RS05855 (MasN3_11470) - 1267339..1267971 (-) 633 WP_281912973.1 hypothetical protein -
  MasN3_RS05860 (MasN3_11480) - 1268081..1269799 (-) 1719 WP_281912975.1 methyl-accepting chemotaxis protein -
  MasN3_RS05865 (MasN3_11490) pilU 1270201..1271337 (-) 1137 WP_281912976.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MasN3_RS05870 (MasN3_11500) pilT 1271347..1272390 (-) 1044 WP_281912978.1 type IV pilus twitching motility protein PilT Machinery gene
  MasN3_RS05875 (MasN3_11510) - 1272448..1273152 (+) 705 WP_281912980.1 YggS family pyridoxal phosphate-dependent enzyme -
  MasN3_RS05880 (MasN3_11520) proC 1273164..1273982 (+) 819 WP_281912981.1 pyrroline-5-carboxylate reductase -
  MasN3_RS05885 (MasN3_11530) - 1274088..1274390 (-) 303 WP_281912982.1 hypothetical protein -
  MasN3_RS05890 (MasN3_11540) - 1274380..1274757 (-) 378 WP_281912983.1 phage holin family protein -
  MasN3_RS05895 (MasN3_11550) - 1274759..1275079 (-) 321 WP_209587824.1 YqjD family protein -
  MasN3_RS05900 (MasN3_11560) ubiA 1275125..1275979 (-) 855 WP_281912984.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41996.40 Da        Isoelectric Point: 6.5060

>NTDB_id=99054 MasN3_RS05865 WP_281912976.1 1270201..1271337(-) (pilU) [Massilia varians strain N-3]
MERDQASKFMFDLLRLMTSKGGSDLFITAGFPPAIKIDGKMTPVSSQALSGTHTADLARAIMNDKQAAGFELTKEANFAI
SPGDLGRFRVSAFMQMGCVGMVLRVITTTIPRFEDLELPETLKDVIMTKRGLVIMVGATGSGKSTTLAAMVGYRNENSYG
HIITIEDPVEFVHPHRNCVITQREVGVDTDSFEAALKNSLRQAPDVIQIGEIRDRETMEHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKGLISQRLIPKKEAKGRVVAMEILLNSPLISDLIFKGDVHEIKEIMKKSRELG
MQTFDQALFDLYEADKISYEDALRNADSVNDLRLNIKLNSKHAKNRDFSTGMDHLGIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=99054 MasN3_RS05865 WP_281912976.1 1270201..1271337(-) (pilU) [Massilia varians strain N-3]
ATGGAACGCGACCAGGCATCCAAGTTCATGTTCGACCTGCTGCGCCTGATGACCAGCAAGGGCGGATCCGATCTGTTCAT
CACCGCGGGCTTCCCGCCGGCGATCAAGATCGATGGCAAGATGACGCCGGTCTCGAGCCAGGCCCTGTCCGGCACCCATA
CGGCCGACCTGGCGCGCGCCATCATGAACGACAAGCAGGCCGCCGGCTTCGAGCTGACCAAGGAAGCGAACTTCGCGATC
AGCCCGGGCGACCTGGGGCGCTTTCGCGTCTCGGCCTTCATGCAGATGGGCTGCGTGGGCATGGTGCTGCGCGTGATCAC
CACCACCATCCCGCGTTTCGAGGACCTCGAGCTGCCGGAAACCCTGAAGGACGTGATCATGACCAAGCGCGGCCTGGTCA
TCATGGTCGGCGCCACCGGCTCGGGCAAATCGACCACGCTGGCGGCGATGGTCGGCTACCGCAACGAGAACAGCTACGGC
CACATCATCACCATCGAGGACCCGGTCGAATTCGTCCACCCGCACCGGAACTGCGTGATCACCCAGCGCGAAGTGGGCGT
CGATACCGACAGTTTCGAGGCGGCGCTGAAGAATTCGCTGCGCCAGGCGCCGGACGTGATCCAGATCGGCGAGATCCGCG
ACCGCGAGACCATGGAGCACGCAATCGCGTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAGCGCC
AACCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCCAGCAACTCCTGATGGACCTCTCGCTCAACCT
GAAAGGCCTGATCTCGCAGCGCCTGATCCCGAAGAAGGAAGCCAAGGGCCGCGTGGTGGCGATGGAGATCCTGCTGAACT
CCCCGCTTATCTCCGACCTGATCTTCAAGGGCGACGTCCACGAGATCAAGGAGATCATGAAGAAGTCGCGCGAACTGGGC
ATGCAAACTTTCGACCAGGCCCTGTTCGACCTGTACGAGGCGGACAAGATCAGCTACGAGGATGCGCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCCTGAACATCAAGCTCAACAGCAAGCACGCGAAGAACCGCGATTTCTCGACCGGGATGGATC
ACCTCGGCATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(101-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

59.664

94.444

0.563
  pilU Vibrio cholerae strain A1552

57.465

93.915

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.373

88.624

0.402
  pilT Pseudomonas aeruginosa PAK

42.985

88.624

0.381
  pilT Acinetobacter baumannii strain A118

43.202

87.566

0.378
  pilT Acinetobacter nosocomialis M2

43.202

87.566

0.378
  pilT Acinetobacter baumannii D1279779

43.202

87.566

0.378
  pilT Pseudomonas stutzeri DSM 10701

41.791

88.624

0.37
  pilT Legionella pneumophila strain Lp02

41.692

87.566

0.365
  pilT Legionella pneumophila strain ERS1305867

41.692

87.566

0.365
  pilT Acinetobacter baylyi ADP1

43.125

84.656

0.365

Multiple sequence alignment