Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R8474_RS09630 Genome accession   NZ_AP026914
Coordinates   1884306..1885247 (-) Length   313 a.a.
NCBI ID   WP_000249549.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700003     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1879306..1890247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8474_RS09590 (PC0003_18730) comYH 1879794..1880747 (-) 954 WP_000345127.1 class I SAM-dependent methyltransferase Machinery gene
  R8474_RS09595 - 1880808..1881402 (-) 595 Protein_1869 class I SAM-dependent methyltransferase -
  R8474_RS09600 (PC0003_18760) comGG/cglG 1881558..1881950 (-) 393 WP_001818028.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8474_RS09605 comGF/cglF 1881928..1882389 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8474_RS09610 (PC0003_18780) comGE/cglE 1882352..1882654 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8474_RS09615 (PC0003_18790) comGD/cglD 1882617..1883021 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8474_RS09620 (PC0003_18800) comGC/cglC 1883014..1883340 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  R8474_RS09625 (PC0003_18810) comGB/cglB 1883342..1884358 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8474_RS09630 (PC0003_18820) comGA/cglA/cilD 1884306..1885247 (-) 942 WP_000249549.1 competence type IV pilus ATPase ComGA Machinery gene
  R8474_RS09635 (PC0003_18830) - 1885323..1885688 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8474_RS09640 (PC0003_18840) - 1885839..1886897 (-) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -
  R8474_RS09645 (PC0003_18850) nagA 1887060..1888211 (-) 1152 WP_001134459.1 N-acetylglucosamine-6-phosphate deacetylase -
  R8474_RS09650 (PC0003_18860) - 1888364..1890181 (-) 1818 WP_001220848.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35543.45 Da        Isoelectric Point: 6.0083

>NTDB_id=97526 R8474_RS09630 WP_000249549.1 1884306..1885247(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700003]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=97526 R8474_RS09630 WP_000249549.1 1884306..1885247(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700003]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment