Detailed information NTG0948528

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V8J88_RS01160
• Genome accession: NZ_CP147776
• Coordinates: 266628..267812 (+)
• Length: 394 a.a.
• NCBI ID: WP_338847310.1
• Uniprot ID: -
• Organism: Massilia sp. W12
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 261628..272812

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V8J88_RS01145 (V8J88_01145)	-	263073..265076 (-)	2004	WP_338847307.1	ATP-binding protein	-
V8J88_RS01150 (V8J88_01150)	-	265169..266272 (-)	1104	WP_338847308.1	ABC transporter substrate-binding protein	-
V8J88_RS01155 (V8J88_01155)	-	266310..266516 (-)	207	WP_338847309.1	hypothetical protein	-
V8J88_RS01160 (V8J88_01160)	pilU	266628..267812 (+)	1185	WP_338847310.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V8J88_RS01165 (V8J88_01165)	-	267967..270297 (-)	2331	WP_338847311.1	EAL domain-containing protein	-
V8J88_RS01170 (V8J88_01170)	-	270433..270756 (+)	324	WP_338847312.1	hypothetical protein	-
V8J88_RS01175 (V8J88_01175)	-	271040..272095 (+)	1056	WP_338847313.1	M23 family metallopeptidase	-

Sequence

Protein

• Length: 394 a.a.
• Molecular weight: 43917.27 Da
• Isoelectric Point: 6.4500

>NTDB_id=948528 V8J88_RS01160 WP_338847310.1 266628..267812(+) (pilU) [Massilia sp. W12]
MSTFGPEEALNYIHQLLKVMHHAGGSDLFISADFPPSMKAQGAMKPLSQQRLTGDITRKLAYALMNDKQKHEFEAEMECN
FAISLPGVCRFRVNVLVQQQQVAMVIRTIASEIPTFEKLDLPPVLKDVIMTKRGLVLVVGGTGSGKSTTLAAMIDYRNTN
SAGHIITVEDPVEYVHRNKGCLITHREVGVDTLSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLHA
NNANQTMDRIINFFPEERRNQLLMDLSSNLRAIISQRLVRTEDGKGRKAAIEILLNTATISDMIMKGNFHGIKEVMHKSR
ELGMCTFDQALYDLYNKGFISYDEAIRNADSANGLRLAIKLNSSRGEPGSQNHAAGPTELNILEEEEPEEEEKK

Nucleotide

• Length: 1185 bp

>NTDB_id=948528 V8J88_RS01160 WP_338847310.1 266628..267812(+) (pilU) [Massilia sp. W12]
ATGTCCACATTCGGCCCCGAAGAGGCGCTAAATTACATCCATCAATTATTGAAAGTGATGCACCATGCGGGCGGTTCGGA
TTTGTTTATCTCGGCTGATTTTCCGCCCAGCATGAAAGCGCAGGGCGCGATGAAGCCGCTCAGTCAGCAGCGCCTGACCG
GGGACATTACCCGCAAGCTGGCGTATGCCTTGATGAACGATAAGCAAAAGCATGAGTTTGAAGCGGAGATGGAATGCAAT
TTCGCCATTTCGCTGCCCGGGGTGTGTCGCTTCCGGGTGAATGTGCTGGTGCAGCAGCAGCAGGTGGCGATGGTGATCCG
CACCATTGCATCGGAGATTCCGACTTTTGAAAAACTTGATCTGCCGCCGGTCTTGAAAGATGTGATCATGACCAAGCGCG
GCCTGGTGCTGGTGGTGGGCGGCACCGGTTCAGGTAAATCGACCACGCTGGCGGCGATGATTGACTACCGCAATACCAAT
TCAGCGGGACATATCATTACTGTAGAGGACCCGGTTGAATATGTGCACCGGAATAAAGGCTGTCTGATTACCCATCGCGA
AGTCGGGGTGGATACCCTGTCCTGGCATCATGCCTTGAAAAACACCTTGCGCCAGGCCCCGGATGTGATTCTGATCGGTG
AGATCCGCGACACGGAAACCATGGAGCATGCGATTGCGTTTGCCGAGACCGGCCATTTATGTCTTGGCACCTTGCATGCG
AACAATGCGAACCAGACTATGGACCGCATCATCAACTTCTTCCCGGAAGAGCGGCGCAATCAATTATTGATGGATCTGTC
CTCAAATTTGCGCGCCATCATTTCGCAGCGTCTGGTGCGCACTGAAGATGGCAAGGGACGCAAGGCGGCGATTGAGATTT
TGCTCAATACCGCCACCATCAGCGATATGATTATGAAGGGCAATTTCCACGGCATCAAAGAAGTCATGCACAAGTCGCGC
GAACTCGGCATGTGCACCTTTGATCAAGCCTTGTACGATTTGTATAACAAAGGCTTTATTTCCTACGACGAAGCGATTCG
CAACGCTGATTCCGCCAATGGCCTGCGTCTGGCGATTAAACTCAACTCCAGCCGGGGCGAACCGGGCAGCCAAAACCATG
CCGCCGGGCCAACGGAATTGAATATTCTGGAGGAAGAAGAGCCGGAAGAGGAGGAAAAGAAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (93-281)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.026	88.579	0.523
pilU	Acinetobacter baylyi ADP1	53.955	89.848	0.485
pilU	Vibrio cholerae strain A1552	50.704	90.102	0.457
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.623	92.893	0.396
pilT	Legionella pneumophila strain Lp02	44.377	83.503	0.371
pilT	Legionella pneumophila strain ERS1305867	44.377	83.503	0.371
pilT	Pseudomonas aeruginosa PAK	43.323	85.533	0.371
pilT	Acinetobacter baylyi ADP1	43.544	84.518	0.368
pilT	Pseudomonas stutzeri DSM 10701	42.73	85.533	0.365
pilT	Acinetobacter baumannii D1279779	43.465	83.503	0.363
pilT	Acinetobacter baumannii strain A118	43.465	83.503	0.363
pilT	Acinetobacter nosocomialis M2	43.465	83.503	0.363