Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V8687_RS16200 Genome accession   NZ_CP146610
Coordinates   3716268..3717380 (-) Length   370 a.a.
NCBI ID   WP_006085705.1    Uniprot ID   -
Organism   Shewanella baltica strain TMP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3711268..3722380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8687_RS16165 (V8687_16155) - 3711760..3712320 (-) 561 WP_006082509.1 DUF4136 domain-containing protein -
  V8687_RS16170 (V8687_16160) - 3712499..3712816 (+) 318 WP_006082510.1 hypothetical protein -
  V8687_RS16175 (V8687_16165) yciH 3712995..3713324 (+) 330 WP_219253466.1 stress response translation initiation inhibitor YciH -
  V8687_RS16180 (V8687_16170) - 3713391..3713954 (+) 564 WP_006082512.1 YqgE/AlgH family protein -
  V8687_RS16185 (V8687_16175) ruvX 3713984..3714409 (+) 426 WP_006082513.1 Holliday junction resolvase RuvX -
  V8687_RS16190 (V8687_16180) hemH 3714495..3715508 (-) 1014 WP_338725494.1 ferrochelatase -
  V8687_RS16195 (V8687_16185) - 3715541..3716080 (-) 540 WP_037392614.1 glutathione peroxidase -
  V8687_RS16200 (V8687_16190) pilU 3716268..3717380 (-) 1113 WP_006085705.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V8687_RS16205 (V8687_16195) pilT 3717390..3718427 (-) 1038 WP_338725495.1 type IV pilus twitching motility protein PilT Machinery gene
  V8687_RS16210 (V8687_16200) - 3718463..3719161 (+) 699 WP_338725496.1 YggS family pyridoxal phosphate-dependent enzyme -
  V8687_RS16215 (V8687_16205) proC 3719377..3720195 (+) 819 WP_006082519.1 pyrroline-5-carboxylate reductase -
  V8687_RS16220 (V8687_16210) - 3720244..3720792 (+) 549 WP_006082520.1 YggT family protein -
  V8687_RS16225 (V8687_16215) yggU 3720792..3721091 (+) 300 WP_006082521.1 DUF167 family protein YggU -
  V8687_RS16230 (V8687_16220) - 3721224..3721658 (+) 435 WP_006082522.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40986.13 Da        Isoelectric Point: 6.2352

>NTDB_id=943360 V8687_RS16200 WP_006085705.1 3716268..3717380(-) (pilU) [Shewanella baltica strain TMP1]
MDVLPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKQEFHSTRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNGHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKESTGSGFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=943360 V8687_RS16200 WP_006085705.1 3716268..3717380(-) (pilU) [Shewanella baltica strain TMP1]
ATGGATGTTCTTCCGTTTTTAAAAGTGATCGTTGAGCGCAAAGCCTCCGACTTGTTCATCACAGCTGGCTTTCCGCCGAG
TGCCAAGATTGACGGTGAATTGCGTCCCTTGGCCGAAAGTGCCTTTACTCCGGCACAATCACTGGATTTTGTTGAATCCT
TAATGACCGAAGCGCAGAAACAAGAGTTTCATTCAACTCGTGAGTGTAACTTTGCTTTTGCTGCAAAGGATTTAGGTCGT
TTTCGGGTGAGCGCCTTTTGGCAGCGTGAATCGCCAGGCTGTGTGATGCGTCGTATCGAAACTAAAATCCCTGACGTCGA
TGATTTAAAACTGCCACCCATTCTGAAAGATCTGGTGATGAGTAAACGTGGTCTCATTATTATGGTGGGCGGAACGGGTA
CAGGTAAGTCGACATCTCTAGCTGCCTTGGTGGGATATCGTAATGGTCATGCTCGAGGCCATATTCTCACGATTGAAGAT
CCAGTCGAATTTGTTCACGACCACAGAAAAAGCATTATCACCCAGCGTGAAGTGGGCATAGATACTGAATCTTTCGATGC
GGCGCTAAAAAGCTCCCTTCGCCAAGCACCCGATGTGATTTTGATTGGTGAAATTCGTACCCAAGAGACGATGGAGTTCG
CTCTGTCGTTTGCGGAAACAGGCCACCTCTGTATGGCAACCTTGCATGCCAACAACGCCAACCAAGCATTAGACCGCATT
ATGCACCTCGTGCCTGAGAGTAAACATCAGCAGTTATTGTTCGATTTGTCATTGAATTTGCGCGGTATTGTGGCGCAGCA
ATTGATCCCAAAAGTCGATGGCACTGGGCGCCGCGCCGCGATTGAAGTATTGATTAACACCCCAAGGGTGGCGAGTTTAA
TTGCGAAGAACGAGCTGCATTTACTCAAAGAAACCATGGCAAAATCCAACGAGCAGGGCATGCAGACGTTCGATCAAGCC
TTGCTCAAACTTTATGTCGATGGCGAGATTAGTTACGCCGATGCGCTGCACCATGCTGATTCACCGAACGATTTACGTTT
GATGATTAAATTACAGAATAAAGAATCCACGGGCTCAGGCTTTATGGAAGGTGTCACCTTAGACATGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.946

100

0.603
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

61.08

95.135

0.581
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.556

97.297

0.395
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362