Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   LLL8_RS04275 Genome accession   NZ_AP025700
Coordinates   863090..863524 (+) Length   144 a.a.
NCBI ID   WP_251900320.1    Uniprot ID   -
Organism   Lactococcus lactis strain L8     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 856749..905854 863090..863524 within 0


Gene organization within MGE regions


Location: 856749..905854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLL8_RS04220 (LLL8_08060) - 856749..857879 (-) 1131 WP_251900309.1 site-specific integrase -
  LLL8_RS04225 (LLL8_08070) - 858010..858711 (-) 702 WP_251900311.1 hypothetical protein -
  LLL8_RS04230 (LLL8_08080) - 858858..859028 (+) 171 WP_251900312.1 hypothetical protein -
  LLL8_RS04235 (LLL8_08090) - 859012..859896 (-) 885 WP_251900313.1 hypothetical protein -
  LLL8_RS04240 (LLL8_08100) - 859954..860541 (-) 588 WP_251900314.1 hypothetical protein -
  LLL8_RS12285 - 860552..860815 (-) 264 WP_350028259.1 hypothetical protein -
  LLL8_RS12290 - 860864..861061 (-) 198 Protein_810 helix-turn-helix transcriptional regulator -
  LLL8_RS04250 (LLL8_08120) - 861262..861489 (+) 228 WP_033900181.1 hypothetical protein -
  LLL8_RS04255 (LLL8_08130) - 861514..862239 (+) 726 WP_251900316.1 ORF6C domain-containing protein -
  LLL8_RS04260 (LLL8_08140) - 862379..862630 (+) 252 WP_033900176.1 hypothetical protein -
  LLL8_RS04265 (LLL8_08150) - 862718..862882 (+) 165 WP_251900317.1 hypothetical protein -
  LLL8_RS04270 (LLL8_08160) - 862879..863100 (+) 222 WP_251900318.1 hypothetical protein -
  LLL8_RS04275 (LLL8_08170) ssb 863090..863524 (+) 435 WP_251900320.1 single-stranded DNA-binding protein Machinery gene
  LLL8_RS04280 (LLL8_08180) - 863535..864431 (+) 897 WP_251900321.1 phage replisome organiser protein -
  LLL8_RS04285 (LLL8_08190) - 864424..864816 (+) 393 WP_251900322.1 hypothetical protein -
  LLL8_RS04290 (LLL8_08200) - 864835..865035 (+) 201 WP_195918835.1 hypothetical protein -
  LLL8_RS04295 (LLL8_08210) - 865032..865286 (+) 255 WP_195918834.1 DUF1031 family protein -
  LLL8_RS04300 (LLL8_08230) - 865414..865650 (+) 237 WP_195918833.1 hypothetical protein -
  LLL8_RS04305 (LLL8_08240) - 865650..865823 (+) 174 WP_251900323.1 hypothetical protein -
  LLL8_RS04310 (LLL8_08250) dcm 865820..867331 (+) 1512 WP_251900324.1 DNA (cytosine-5-)-methyltransferase -
  LLL8_RS04315 (LLL8_08260) - 867400..867666 (+) 267 WP_195918956.1 hypothetical protein -
  LLL8_RS04320 (LLL8_08270) - 867663..867857 (+) 195 WP_251900325.1 DUF1660 domain-containing protein -
  LLL8_RS04325 (LLL8_08280) - 867890..868282 (+) 393 WP_251900326.1 RusA family crossover junction endodeoxyribonuclease -
  LLL8_RS04330 (LLL8_08290) - 868400..868744 (+) 345 WP_251900327.1 hypothetical protein -
  LLL8_RS04335 (LLL8_08300) - 868790..869134 (+) 345 WP_251900328.1 hypothetical protein -
  LLL8_RS04340 (LLL8_08310) - 869251..869565 (+) 315 WP_251900329.1 hypothetical protein -
  LLL8_RS04345 (LLL8_08320) - 869642..869941 (+) 300 WP_039116109.1 hypothetical protein -
  LLL8_RS04350 (LLL8_08330) - 869990..870289 (+) 300 WP_251900330.1 HNH endonuclease -
  LLL8_RS04355 (LLL8_08340) - 870430..871023 (+) 594 WP_251900331.1 helix-turn-helix transcriptional regulator -
  LLL8_RS04360 (LLL8_08350) - 871020..872759 (+) 1740 WP_180350364.1 terminase large subunit -
  LLL8_RS04365 (LLL8_08360) - 872951..874144 (+) 1194 WP_227150006.1 phage portal protein -
  LLL8_RS04370 (LLL8_08370) - 874131..874916 (+) 786 WP_058210168.1 head maturation protease, ClpP-related -
  LLL8_RS04375 (LLL8_08380) - 874916..876043 (+) 1128 WP_227150005.1 phage major capsid protein -
  LLL8_RS04380 (LLL8_08390) - 876104..876361 (+) 258 WP_227150004.1 hypothetical protein -
  LLL8_RS04385 (LLL8_08400) - 876379..876711 (+) 333 WP_058210171.1 hypothetical protein -
  LLL8_RS04390 (LLL8_08410) - 876677..877063 (+) 387 WP_227150003.1 phage head-tail adapter protein -
  LLL8_RS04395 (LLL8_08420) - 877056..877460 (+) 405 WP_227150002.1 HK97 gp10 family phage protein -
  LLL8_RS04400 (LLL8_08430) - 877457..877846 (+) 390 WP_227150001.1 hypothetical protein -
  LLL8_RS04405 (LLL8_08440) - 877861..878442 (+) 582 WP_227150000.1 major tail protein -
  LLL8_RS04410 (LLL8_08450) - 878508..878813 (+) 306 WP_227149999.1 hypothetical protein -
  LLL8_RS12240 (LLL8_08460) - 878894..879016 (+) 123 WP_255217742.1 hypothetical protein -
  LLL8_RS04415 (LLL8_08470) - 879117..879914 (+) 798 WP_227149998.1 FxLYD domain-containing protein -
  LLL8_RS04420 (LLL8_08480) - 880024..883995 (+) 3972 WP_227149997.1 phage tail tape measure protein -
  LLL8_RS04425 (LLL8_08490) - 883992..885632 (+) 1641 WP_251900332.1 distal tail protein Dit -
  LLL8_RS04430 (LLL8_08500) - 885632..889021 (+) 3390 WP_227149995.1 phage tail spike protein -
  LLL8_RS04435 (LLL8_08510) - 889033..889263 (+) 231 WP_227149994.1 hypothetical protein -
  LLL8_RS04440 (LLL8_08520) - 889276..889692 (+) 417 WP_227149993.1 phage holin family protein -
  LLL8_RS04445 (LLL8_08530) - 889689..890036 (+) 348 WP_227149992.1 phage holin -
  LLL8_RS04450 (LLL8_08540) - 890033..890764 (+) 732 WP_227149991.1 peptidoglycan amidohydrolase family protein -
  LLL8_RS04455 (LLL8_08550) - 890794..891135 (-) 342 WP_227149990.1 MmcQ/YjbR family DNA-binding protein -
  LLL8_RS04460 (LLL8_08560) - 891289..891951 (+) 663 WP_251900333.1 hypothetical protein -
  LLL8_RS04465 (LLL8_08570) - 891941..893071 (+) 1131 WP_227149988.1 ImmA/IrrE family metallo-endopeptidase -
  LLL8_RS04470 (LLL8_08580) - 894184..894384 (+) 201 WP_014570681.1 cold-shock protein -
  LLL8_RS04475 (LLL8_08590) - 894941..895375 (+) 435 WP_227149987.1 YfbU family protein -
  LLL8_RS04480 (LLL8_08600) - 895969..896478 (+) 510 WP_033900119.1 hypothetical protein -
  LLL8_RS04485 (LLL8_08610) - 896471..897799 (+) 1329 WP_033900116.1 glycosyltransferase family 2 protein -
  LLL8_RS04490 (LLL8_08620) - 898027..898563 (+) 537 WP_010905603.1 HdeD family acid-resistance protein -
  LLL8_RS04495 (LLL8_08630) pnuC 898760..899491 (+) 732 WP_058203634.1 nicotinamide riboside transporter PnuC -
  LLL8_RS04500 (LLL8_08640) - 899606..899761 (-) 156 WP_003131383.1 hypothetical protein -
  LLL8_RS04505 (LLL8_08650) - 900019..900444 (+) 426 WP_058203635.1 universal stress protein -
  LLL8_RS04510 (LLL8_08660) - 900566..900913 (+) 348 WP_058203636.1 HU family DNA-binding protein -
  LLL8_RS04515 (LLL8_08670) - 900981..901514 (-) 534 WP_003131387.1 TetR/AcrR family transcriptional regulator -
  LLL8_RS04520 (LLL8_08680) - 901678..904077 (+) 2400 WP_251900334.1 YhgE/Pip domain-containing protein -
  LLL8_RS04525 (LLL8_08690) - 904263..904595 (+) 333 WP_003131392.1 metal-sulfur cluster assembly factor -
  LLL8_RS04530 (LLL8_08700) - 905075..905854 (-) 780 WP_017864372.1 ABC transporter ATP-binding protein -

Sequence


Protein


Download         Length: 144 a.a.        Molecular weight: 16207.89 Da        Isoelectric Point: 4.7294

>NTDB_id=93683 LLL8_RS04275 WP_251900320.1 863090..863524(+) (ssb) [Lactococcus lactis strain L8]
MINNVVLVGRITRDPELRYTPQQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAENLANWAKKGALIGVTGRIQT
RSYENQQGQRVYITEVVADSFQMLESKGGTKQNEESQNNTAPNFARNDSNDIPGTEISDDDLPF

Nucleotide


Download         Length: 435 bp        

>NTDB_id=93683 LLL8_RS04275 WP_251900320.1 863090..863524(+) (ssb) [Lactococcus lactis strain L8]
ATGATAAATAACGTTGTACTAGTTGGACGTATTACACGAGATCCAGAATTAAGATATACACCACAACAAAATCAGGCAGT
TGCAACTTTCAGTTTGGCTGTTAATCGTCAATTTAAGAACGCAAACGGAGAACGTGAAGCAGACTTCATAAATTGCGTTA
TCTGGCGTCAACAAGCCGAAAATTTAGCAAATTGGGCTAAAAAAGGGGCTTTGATTGGAGTAACTGGCAGAATCCAAACA
CGAAGTTATGAAAATCAGCAAGGACAACGAGTCTACATCACAGAAGTAGTTGCTGACAGTTTTCAAATGCTGGAAAGCAA
AGGCGGAACAAAGCAAAATGAAGAGTCACAAAATAATACAGCTCCGAATTTTGCGCGTAATGATTCCAATGATATTCCAG
GCACAGAAATTAGTGATGATGATTTGCCATTTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

54.335

100

0.653

  ssbA Bacillus subtilis subsp. subtilis str. 168

52.542

100

0.646

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.879

74.306

0.438

  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.056

100

0.431

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.361

100

0.424

  ssbB/cilA Streptococcus mitis NCTC 12261

41.667

100

0.417

  ssbB/cilA Streptococcus pneumoniae Rx1

41.667

100

0.417

  ssbB/cilA Streptococcus pneumoniae D39

41.667

100

0.417

  ssbB/cilA Streptococcus pneumoniae R6

41.667

100

0.417

  ssbB/cilA Streptococcus mitis SK321

41.667

100

0.417

  ssbA Streptococcus mutans UA159

38.889

100

0.389

  ssb Neisseria meningitidis MC58

31.034

100

0.375


Multiple sequence alignment