Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V5G16_RS01780 Genome accession   NZ_CP145062
Coordinates   342703..343833 (+) Length   376 a.a.
NCBI ID   WP_082285182.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain WHO_T_2024     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 337703..348833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5G16_RS01755 (V5G16_01760) - 338149..338298 (+) 150 WP_003687747.1 hypothetical protein -
  V5G16_RS01760 (V5G16_01765) - 338522..338629 (+) 108 Protein_343 IS5/IS1182 family transposase -
  V5G16_RS01765 (V5G16_01770) lepB 338734..339753 (-) 1020 WP_003706641.1 signal peptidase I -
  V5G16_RS01770 (V5G16_01775) lepA 339899..341692 (-) 1794 WP_003687751.1 translation elongation factor 4 -
  V5G16_RS01775 (V5G16_01780) - 341834..342535 (-) 702 WP_003692750.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  V5G16_RS01780 (V5G16_01785) pilU 342703..343833 (+) 1131 WP_082285182.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V5G16_RS01785 (V5G16_01790) - 343867..344844 (+) 978 WP_003696824.1 DNA polymerase III subunit delta' -
  V5G16_RS01790 (V5G16_01795) - 344848..345198 (+) 351 WP_003687757.1 PilZ domain-containing protein -
  V5G16_RS01795 (V5G16_01800) - 345203..345982 (+) 780 WP_003690796.1 TatD family hydrolase -
  V5G16_RS01800 (V5G16_01805) - 346031..346222 (+) 192 WP_228547134.1 DUF333 domain-containing protein -
  V5G16_RS01805 (V5G16_01810) grxD 346323..346634 (+) 312 WP_003687762.1 Grx4 family monothiol glutaredoxin -
  V5G16_RS01810 (V5G16_01815) upp 346740..347366 (+) 627 WP_003687763.1 uracil phosphoribosyltransferase -
  V5G16_RS01815 (V5G16_01820) - 347389..347709 (+) 321 WP_003687765.1 DUF2322 family protein -
  V5G16_RS01820 (V5G16_01825) - 347878..348519 (+) 642 Protein_355 IS1595 family transposase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42227.37 Da        Isoelectric Point: 6.2891

>NTDB_id=936695 V5G16_RS01780 WP_082285182.1 342703..343833(+) (pilU) [Neisseria gonorrhoeae strain WHO_T_2024]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGIGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRRAQSSDPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=936695 V5G16_RS01780 WP_082285182.1 342703..343833(+) (pilU) [Neisseria gonorrhoeae strain WHO_T_2024]
ATGTTTACCGATGAAAATATGACTGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCTATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGTGCGACGGCGTTGGTATTCCG
CGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGCCTTGAAGGATGTTGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCATCGGCTCGGGCAAATCGACTTCGCTCGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGATCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATTACCCAGCGCGA
GGTCGGCGTGGACACGGAAAACTGGATGGCGGCGTTGAAAAATACGCTGCGTCAGGCGCCGGATGTGATCCTTATCGGCG
AAATCCGCGACCGTGAAACAATGGACTACGCCATCGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGAGATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCCGCACATGATTTGCGTTTGGCGGTACAGTTGCGCAGCCGCAGGGCACAAAGTTCCGACCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.375

93.617

0.556
  pilU Acinetobacter baylyi ADP1

57.434

91.223

0.524
  pilU Vibrio cholerae strain A1552

54.085

94.415

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.059

93.883

0.404
  pilT Legionella pneumophila strain Lp02

43.519

86.17

0.375
  pilT Legionella pneumophila strain ERS1305867

43.519

86.17

0.375
  pilT Acinetobacter baylyi ADP1

42.424

87.766

0.372
  pilT Acinetobacter nosocomialis M2

42.593

86.17

0.367
  pilT Acinetobacter baumannii D1279779

42.593

86.17

0.367
  pilT Acinetobacter baumannii strain A118

42.593

86.17

0.367
  pilT Pseudomonas aeruginosa PAK

42.073

87.234

0.367
  pilT Neisseria meningitidis 8013

40.597

89.096

0.362