Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V5G17_RS02145 Genome accession   NZ_CP145024
Coordinates   414513..415643 (+) Length   376 a.a.
NCBI ID   WP_106333318.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain WHO_U_2024     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409513..420643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V5G17_RS02120 (V5G17_02125) - 409959..410108 (+) 150 WP_003687747.1 hypothetical protein -
  V5G17_RS02125 (V5G17_02130) - 410332..410439 (+) 108 Protein_410 IS5/IS1182 family transposase -
  V5G17_RS02130 (V5G17_02135) lepB 410544..411563 (-) 1020 WP_003706641.1 signal peptidase I -
  V5G17_RS02135 (V5G17_02140) lepA 411709..413502 (-) 1794 WP_003687751.1 translation elongation factor 4 -
  V5G17_RS02140 (V5G17_02145) - 413644..414345 (-) 702 WP_003692750.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  V5G17_RS02145 (V5G17_02150) pilU 414513..415643 (+) 1131 WP_106333318.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V5G17_RS02150 (V5G17_02155) - 415677..416654 (+) 978 WP_003687755.1 DNA polymerase III subunit delta' -
  V5G17_RS02155 (V5G17_02160) - 416658..417008 (+) 351 WP_003687757.1 PilZ domain-containing protein -
  V5G17_RS02160 (V5G17_02165) - 417013..417792 (+) 780 WP_003690796.1 TatD family hydrolase -
  V5G17_RS02165 (V5G17_02170) - 417818..418033 (+) 216 WP_003690799.1 DUF333 domain-containing protein -
  V5G17_RS02170 (V5G17_02175) grxD 418134..418445 (+) 312 WP_003687762.1 Grx4 family monothiol glutaredoxin -
  V5G17_RS02175 (V5G17_02180) upp 418551..419177 (+) 627 WP_003690802.1 uracil phosphoribosyltransferase -
  V5G17_RS02180 (V5G17_02185) - 419200..419520 (+) 321 WP_003690805.1 DUF2322 family protein -
  V5G17_RS02185 (V5G17_02190) - 419689..420330 (+) 642 Protein_422 IS1595 family transposase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42230.29 Da        Isoelectric Point: 6.2365

>NTDB_id=936012 V5G17_RS02145 WP_106333318.1 414513..415643(+) (pilU) [Neisseria gonorrhoeae strain WHO_U_2024]
MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECN
FAISLPDTSRFRVNAMIQRGATALVFHAITSKIPKFESLNLPPALKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHA
NSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKST
TLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRFAVQLRSRRAQSSDPDLELL

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=936012 V5G17_RS02145 WP_106333318.1 414513..415643(+) (pilU) [Neisseria gonorrhoeae strain WHO_U_2024]
ATGTTTACCGATGAAAATATGACTGCAAAGGAAGAACTGTTCGCATGGCTGCGCCATATGAACAAAAACAAAGGTTCCGA
CCTGTTTGTGACGACCCATTTCCCGCCCGCTATGAAGCTGGACGGCAAAATCACCCGCATCACGGACGAACCGCTGACGG
CGGAAAAATGTATGGAAATCGCCTTTTCGATTATGAGTGCGAAGCAGGCGGAAGAATTTTCATCGACCAACGAGTGCAAC
TTCGCCATCAGCCTGCCGGACACCAGCCGCTTCCGCGTCAATGCGATGATACAGCGCGGTGCGACGGCGTTGGTATTCCA
CGCGATTACCAGCAAGATTCCCAAGTTTGAAAGCCTGAACCTGCCGCCGGCCTTGAAGGATGTTGCGCTGAAAAAACGCG
GGCTGGTTATTTTTGTCGGCGGCACCGGCTCGGGCAAATCGACTTCGCTCGCCTCGCTTATCGACTACCGCAATGAAAAT
TCGTTCGGACACATCATCACCATCGAAGATCCGATCGAGTTTGTCCACGAACACAAAAACTGCATCATTACCCAGCGCGA
GGTCGGCGTGGACACGGAAAACTGGATGGCGGCGTTGAAAAATACGCTGCGTCAGGCGCCGGATGTGATCCTTATCGGCG
AAATCCGCGACCGTGAAACAATGGACTACGCCATCGCCTTTGCCGAAACGGGGCATTTGTGTATGGCGACGCTGCACGCC
AACAGCACCAATCAGGCGCTCGACCGCATCATCAACTTCTTCCCCGAGGAGCGGCGCGAACAATTGCTGACGGATTTGTC
GCTCAACCTTCAGGCGTTTATTTCGCAACGCCTCGTTCCGCGAGACGGCGGCAAGGGCAGGGTGGCGGCAGTCGAGGTGC
TGCTCAATTCGCCCCTGATTTCGGAGTTGATTCACAACGGCAACATCCATGAAATCAAAGAAGTGATGAAAAAATCCACT
ACCCTGGGTATGCAGACCTTCGACCAACACCTTTACCAATTGTATGAAAAAGGCGAGATTTCCTTGCAGGATGCCTTGAA
AAATGCCGATTCCGCACATGATTTGCGTTTTGCGGTACAGTTGCGCAGCCGCAGGGCACAAAGTTCCGACCCCGATTTGG
AACTGCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-279)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.091

93.617

0.553
  pilU Acinetobacter baylyi ADP1

57.143

91.223

0.521
  pilU Vibrio cholerae strain A1552

53.803

94.415

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.149

91.223

0.394
  pilT Legionella pneumophila strain Lp02

43.519

86.17

0.375
  pilT Legionella pneumophila strain ERS1305867

43.519

86.17

0.375
  pilT Acinetobacter baylyi ADP1

42.424

87.766

0.372
  pilT Acinetobacter nosocomialis M2

42.593

86.17

0.367
  pilT Acinetobacter baumannii D1279779

42.593

86.17

0.367
  pilT Acinetobacter baumannii strain A118

42.593

86.17

0.367
  pilT Pseudomonas aeruginosa PAK

42.073

87.234

0.367
  pilT Neisseria meningitidis 8013

40.653

89.628

0.364