Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA1   Type   Machinery gene
Locus tag   VWP12_RS01425 Genome accession   NZ_CP144142
Coordinates   296486..296929 (-) Length   147 a.a.
NCBI ID   WP_410474925.1    Uniprot ID   -
Organism   Guyparkeria sp. TX1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 291486..301929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VWP12_RS01405 (VWP12_01405) - 291600..292838 (+) 1239 WP_410474289.1 AmpG family muropeptide MFS transporter -
  VWP12_RS01410 (VWP12_01410) - 292924..293409 (+) 486 WP_125199637.1 hypothetical protein -
  VWP12_RS01415 (VWP12_01415) - 293644..294447 (+) 804 WP_410474290.1 ProQ/FINO family protein -
  VWP12_RS01420 (VWP12_01420) mtgA 295269..295982 (+) 714 WP_410474291.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  VWP12_RS01425 (VWP12_01425) pilA/pilA1 296486..296929 (-) 444 WP_410474925.1 pilin Machinery gene
  VWP12_RS01430 (VWP12_01430) - 297233..297511 (-) 279 WP_410474292.1 ComEA family DNA-binding protein -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15192.00 Da        Isoelectric Point: 3.9013

>NTDB_id=932491 VWP12_RS01425 WP_410474925.1 296486..296929(-) (pilA/pilA1) [Guyparkeria sp. TX1]
MNKQVQKGFTLIELMIVVAIIGILAAIALPAYQDYTARAQASEGFTSTAGLRADIAVFSSENGTLTGVGSDSAITTAASE
LNGKYFSTVAVATDGTGTITVTFDDGVLAGITDSNLTITPNVETSGQISSWDCDGLEDKYLPSACRP

Nucleotide


Download         Length: 444 bp        

>NTDB_id=932491 VWP12_RS01425 WP_410474925.1 296486..296929(-) (pilA/pilA1) [Guyparkeria sp. TX1]
ATGAACAAGCAGGTTCAGAAGGGTTTTACCCTGATCGAGCTGATGATCGTCGTGGCGATTATCGGTATCTTGGCTGCCAT
CGCGCTGCCGGCATACCAAGATTACACCGCTCGTGCGCAGGCGTCCGAGGGTTTCACCTCCACTGCGGGTCTTCGTGCAG
ACATCGCTGTGTTTTCCTCTGAGAATGGCACATTGACAGGTGTCGGTAGCGATTCTGCCATCACCACTGCGGCTAGTGAG
CTGAACGGGAAGTATTTCAGCACTGTTGCGGTTGCTACTGACGGCACCGGTACGATCACGGTGACTTTTGATGACGGTGT
GCTTGCTGGTATCACTGATTCGAACTTGACAATTACACCTAACGTTGAGACGTCGGGCCAGATCTCCAGTTGGGATTGTG
ATGGTTTGGAAGATAAGTATCTTCCGAGTGCATGCCGACCGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA1 Eikenella corrodens VA1

52.564

100

0.558
  comP Acinetobacter baylyi ADP1

44.805

100

0.469
  pilA Ralstonia pseudosolanacearum GMI1000

39.412

100

0.456
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

33.69

100

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.611

100

0.422
  pilE Neisseria gonorrhoeae strain FA1090

38.509

100

0.422
  pilE Neisseria gonorrhoeae MS11

37.888

100

0.415
  pilA2 Legionella pneumophila strain ERS1305867

40.972

97.959

0.401
  pilA2 Legionella pneumophila str. Paris

40.972

97.959

0.401
  pilA Pseudomonas aeruginosa PAK

36.842

100

0.381
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.5

97.959

0.367
  pilA Acinetobacter baumannii strain A118

37.589

95.918

0.361