Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VNN36_RS03315 Genome accession   NZ_CP141717
Coordinates   663778..664722 (-) Length   314 a.a.
NCBI ID   WP_339014497.1    Uniprot ID   -
Organism   Lactococcus garvieae strain R22-8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 658778..669722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN36_RS03280 (VNN36_03280) - 659386..660255 (+) 870 WP_339014029.1 RluA family pseudouridine synthase -
  VNN36_RS03285 (VNN36_03285) - 660341..661186 (-) 846 WP_003133722.1 zinc ABC transporter substrate-binding protein -
  VNN36_RS03290 (VNN36_03290) comYF 661419..661859 (-) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  VNN36_RS03295 (VNN36_03295) comGE 661840..662073 (-) 234 WP_339014031.1 competence type IV pilus minor pilin ComGE -
  VNN36_RS03300 (VNN36_03300) comGD 662105..662521 (-) 417 WP_003133718.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN36_RS03305 (VNN36_03305) comYC 662496..662801 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN36_RS03310 (VNN36_03310) comGB 662820..663839 (-) 1020 WP_339014494.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN36_RS03315 (VNN36_03315) comGA 663778..664722 (-) 945 WP_339014497.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN36_RS03320 (VNN36_03320) rpsU 664910..665086 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN36_RS03325 (VNN36_03325) guaB 665209..666690 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN36_RS03330 (VNN36_03330) - 666859..668460 (+) 1602 WP_339014034.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35806.08 Da        Isoelectric Point: 6.9762

>NTDB_id=918763 VNN36_RS03315 WP_339014497.1 663778..664722(-) (comGA) [Lactococcus garvieae strain R22-8]
MIQVLAKNLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELNLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQSQLHFWNSEILKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAREKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=918763 VNN36_RS03315 WP_339014497.1 663778..664722(-) (comGA) [Lactococcus garvieae strain R22-8]
ATGATACAGGTTTTGGCAAAAAACCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCAATCTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGTACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAGCCAACTTCATTTTTGGAATTCAGAGATTTTGAAATCTTTTATTGGTGGGAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTCATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCCGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTTAATAAAGTAATAGGGAATGATTACGA
TGCCTTGATTAAGCTCTCTCTTCGCCACAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAGACGGCAAAAG
CTGTTCTCCGCGCAAGTTTGACGGGGTATACCGTGTTTTCTACTGTTCATGCACGCTCAATATCGGGTGTAATTGCACGG
CTGAAGGAACTGGGGTTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAACGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCGCGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.51

100

0.605
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.035

99.682

0.529
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.412

99.045

0.519
  comYA Streptococcus mutans UA159

51.282

99.363

0.51
  comYA Streptococcus mutans UA140

51.282

99.363

0.51