Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   VNN36_RS03290 Genome accession   NZ_CP141717
Coordinates   661419..661859 (-) Length   146 a.a.
NCBI ID   WP_014024179.1    Uniprot ID   -
Organism   Lactococcus garvieae strain R22-8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 656419..666859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN36_RS03275 (VNN36_03275) - 657022..659310 (-) 2289 WP_176490035.1 PBP1A family penicillin-binding protein -
  VNN36_RS03280 (VNN36_03280) - 659386..660255 (+) 870 WP_339014029.1 RluA family pseudouridine synthase -
  VNN36_RS03285 (VNN36_03285) - 660341..661186 (-) 846 WP_003133722.1 zinc ABC transporter substrate-binding protein -
  VNN36_RS03290 (VNN36_03290) comYF 661419..661859 (-) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  VNN36_RS03295 (VNN36_03295) comGE 661840..662073 (-) 234 WP_339014031.1 competence type IV pilus minor pilin ComGE -
  VNN36_RS03300 (VNN36_03300) comGD 662105..662521 (-) 417 WP_003133718.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN36_RS03305 (VNN36_03305) comYC 662496..662801 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN36_RS03310 (VNN36_03310) comGB 662820..663839 (-) 1020 WP_339014494.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN36_RS03315 (VNN36_03315) comGA 663778..664722 (-) 945 WP_339014497.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN36_RS03320 (VNN36_03320) rpsU 664910..665086 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN36_RS03325 (VNN36_03325) guaB 665209..666690 (-) 1482 WP_003133714.1 IMP dehydrogenase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 16835.45 Da        Isoelectric Point: 9.6906

>NTDB_id=918759 VNN36_RS03290 WP_014024179.1 661419..661859(-) (comYF) [Lactococcus garvieae strain R22-8]
MFLKKNKVTAFTLLESLLALLVLVGTFSLFLGMTKMFHEEVKRATTDHTQDWQLFCSLLRSELEGASLDKVENNYLYVRK
HVNLRFGLSSQGDFRKTNANGRGYQPMIHHLKNAKISQEGEQIKIILTFEKGGDRTFLYTFPEKES

Nucleotide


Download         Length: 441 bp        

>NTDB_id=918759 VNN36_RS03290 WP_014024179.1 661419..661859(-) (comYF) [Lactococcus garvieae strain R22-8]
TTGTTCTTGAAAAAAAATAAAGTTACAGCCTTTACACTTTTGGAATCACTCCTTGCTTTACTCGTGTTAGTGGGCACTTT
TTCTCTTTTTCTAGGTATGACTAAAATGTTTCATGAGGAAGTGAAACGTGCTACAACAGACCATACCCAAGACTGGCAAC
TTTTTTGTAGTCTACTGAGAAGTGAACTTGAGGGAGCAAGTTTAGATAAGGTAGAAAATAACTATCTTTATGTACGCAAA
CATGTTAACCTAAGGTTTGGCCTTTCCTCTCAAGGGGATTTTCGAAAAACTAACGCGAATGGGCGAGGCTATCAGCCCAT
GATTCATCATTTAAAAAATGCAAAAATCAGCCAAGAGGGAGAGCAGATAAAAATTATCTTGACTTTTGAAAAAGGAGGAG
ACCGCACATTTCTTTACACGTTCCCAGAGAAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

50.355

96.575

0.486
  comYF Streptococcus mutans UA159

49.645

96.575

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

49.64

95.205

0.473
  comGF/cglF Streptococcus pneumoniae TIGR4

40.972

98.63

0.404
  comGF/cglF Streptococcus pneumoniae D39

40.972

98.63

0.404
  comGF/cglF Streptococcus pneumoniae Rx1

40.972

98.63

0.404
  comGF/cglF Streptococcus pneumoniae R6

40.972

98.63

0.404
  comGF/cglF Streptococcus mitis NCTC 12261

38.889

98.63

0.384
  comGF/cglF Streptococcus mitis SK321

38.194

98.63

0.377