Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   VNN36_RS03310 Genome accession   NZ_CP141717
Coordinates   662820..663839 (-) Length   339 a.a.
NCBI ID   WP_339014494.1    Uniprot ID   -
Organism   Lactococcus garvieae strain R22-8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 657820..668839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN36_RS03280 (VNN36_03280) - 659386..660255 (+) 870 WP_339014029.1 RluA family pseudouridine synthase -
  VNN36_RS03285 (VNN36_03285) - 660341..661186 (-) 846 WP_003133722.1 zinc ABC transporter substrate-binding protein -
  VNN36_RS03290 (VNN36_03290) comYF 661419..661859 (-) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  VNN36_RS03295 (VNN36_03295) comGE 661840..662073 (-) 234 WP_339014031.1 competence type IV pilus minor pilin ComGE -
  VNN36_RS03300 (VNN36_03300) comGD 662105..662521 (-) 417 WP_003133718.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN36_RS03305 (VNN36_03305) comYC 662496..662801 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN36_RS03310 (VNN36_03310) comGB 662820..663839 (-) 1020 WP_339014494.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN36_RS03315 (VNN36_03315) comGA 663778..664722 (-) 945 WP_339014497.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN36_RS03320 (VNN36_03320) rpsU 664910..665086 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN36_RS03325 (VNN36_03325) guaB 665209..666690 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN36_RS03330 (VNN36_03330) - 666859..668460 (+) 1602 WP_339014034.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39395.15 Da        Isoelectric Point: 9.8954

>NTDB_id=918762 VNN36_RS03310 WP_339014494.1 662820..663839(-) (comGB) [Lactococcus garvieae strain R22-8]
MDLSQLLQLRAKKLSLAKQIKLIQLMNKLFKSGFHLSEIIDFLERSGLTEAYFISKMRAGLLNGQSFSGILAKLKFNEDI
VTQLSLAESHGNVEYTLGLIEEKLRRVLNIRKKLIQVATYPLVLLAFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPTL
FLGCLFSLLVFFYLGRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDRGQKFEDALRFYPIFTKELALIVEYGELKSKLGKELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=918762 VNN36_RS03310 WP_339014494.1 662820..663839(-) (comGB) [Lactococcus garvieae strain R22-8]
ATGGATTTATCTCAGCTGTTACAGCTGCGCGCGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTATTCAACTTATGAA
TAAGCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAAAGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGCAGGTTTACTCAACGGGCAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTAATGAGGATATT
GTGACTCAACTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACTTTAGGGTTGATTGAAGAAAAACTGAGACGCGT
TTTGAACATTCGCAAAAAGCTCATCCAAGTTGCCACCTACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GGTTGAAAAATTATTTACTGCCTCAACTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGACCCTC
TTTTTAGGATGCCTCTTCAGTTTGCTTGTTTTCTTTTATCTGGGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTTGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCAAATATTAGATAGGGGTCAGAAGTTTGAAGACGCCCTTCGATTTTATCCCATATTTACCAA
GGAGTTGGCATTAATTGTAGAATATGGTGAACTAAAGTCTAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.846

99.705

0.537
  comGB/cglB Streptococcus mitis NCTC 12261

53.134

98.82

0.525
  comGB/cglB Streptococcus pneumoniae D39

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae R6

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

52.537

98.82

0.519
  comGB/cglB Streptococcus mitis SK321

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae Rx1

52.537

98.82

0.519
  comYB Streptococcus gordonii str. Challis substr. CH1

52.083

99.115

0.516
  comYB Streptococcus mutans UA140

51.266

93.215

0.478
  comYB Streptococcus mutans UA159

50.949

93.215

0.475