Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U3649_RS18150 Genome accession   NZ_CP141262
Coordinates   4157244..4158368 (+) Length   374 a.a.
NCBI ID   WP_407352690.1    Uniprot ID   -
Organism   Luteimonas sp. R10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4152244..4163368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U3649_RS18125 (U3649_18025) - 4152450..4153637 (-) 1188 WP_407352686.1 cystathionine gamma-synthase -
  U3649_RS18130 (U3649_18030) - 4153923..4155299 (-) 1377 WP_407352687.1 pyridoxal-phosphate dependent enzyme -
  U3649_RS18135 (U3649_18035) - 4155429..4155647 (-) 219 WP_407354655.1 YdcH family protein -
  U3649_RS18140 (U3649_18040) - 4156177..4156770 (-) 594 WP_407352688.1 hypothetical protein -
  U3649_RS18145 (U3649_18045) - 4156772..4157131 (-) 360 WP_407352689.1 DUF4398 domain-containing protein -
  U3649_RS18150 (U3649_18050) pilU 4157244..4158368 (+) 1125 WP_407352690.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U3649_RS18155 (U3649_18055) maiA 4158576..4159238 (-) 663 WP_407352691.1 maleylacetoacetate isomerase -
  U3649_RS18160 (U3649_18060) - 4159360..4160349 (-) 990 WP_407352692.1 fumarylacetoacetate hydrolase family protein -
  U3649_RS18165 (U3649_18065) - 4160683..4162266 (+) 1584 WP_407352693.1 YadA family autotransporter adhesin -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41580.78 Da        Isoelectric Point: 5.6229

>NTDB_id=916514 U3649_RS18150 WP_407352690.1 4157244..4158368(+) (pilU) [Luteimonas sp. R10]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPTGMVKKIAYSLMDEGQVPVFERELELNMALSLQDSGR
FRVNVFKQRGEIGMVIRAIRSVIPSIEELQLPQALKQIIMAPRGLVLIVGSTGSGKSTTLASMIDYRNSNTSGHILTIED
PIEYLHKHKKSIVNQREVGLDTHNFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFNESAHKNVLMNLALNLKAVVSQRLVVGVDGRRLPAAEVLINTPMIRDLLRRGQVHEIKQAMEETLEEGMESFDQCL
FRLYKEGKIEMEEALKAADSRDGLALKFRLSEGGTGEHDPYADVFDSASENEHT

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=916514 U3649_RS18150 WP_407352690.1 4157244..4158368(+) (pilU) [Luteimonas sp. R10]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAAAACGCCTCGGACATGTTCCTGACCACCGGGGCGCCGGTCTA
CATCAAGGTCGAGGGCAGACTGTACCCGCTCGGAAACACCGGGCTGCCCACCGGCATGGTGAAGAAGATCGCCTATTCGC
TCATGGACGAGGGCCAGGTGCCGGTGTTCGAGCGCGAGCTGGAGCTCAACATGGCACTGTCGCTGCAGGATTCGGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGATCGGCATGGTGATCCGCGCCATCCGCAGCGTCATCCCCAGCATCGA
GGAACTGCAGCTGCCGCAGGCGCTCAAGCAGATCATCATGGCCCCGCGCGGGCTGGTGTTGATCGTCGGCTCCACCGGGT
CGGGCAAGTCGACCACGCTGGCGTCGATGATCGACTATCGCAACAGCAACACCTCGGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGAAATCGATCGTCAACCAGCGCGAGGTCGGGCTCGACACCCACAACTTCCACAA
CGCGCTGAAGAACGCCATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAGATCCTCGACGCGACCACCATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCAACGAATCCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTCAAGGCCGTGGTCAGCCAGCG
CCTGGTGGTCGGCGTCGACGGCCGCCGGTTGCCCGCGGCCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGACAGGTGCACGAGATCAAGCAGGCGATGGAGGAGACCCTGGAGGAAGGCATGGAAAGCTTCGACCAGTGCCTG
TTCCGGCTCTACAAGGAAGGCAAGATCGAGATGGAGGAGGCGCTCAAGGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGGCTGTCGGAGGGCGGGACCGGCGAGCACGACCCCTATGCCGACGTCTTCGACAGCGCGTCGGAGAACGAGCACA
CCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.253

95.989

0.492
  pilU Acinetobacter baylyi ADP1

48.87

94.652

0.463
  pilU Vibrio cholerae strain A1552

48.305

94.652

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.236

100

0.436
  pilT Acinetobacter nosocomialis M2

41.42

90.374

0.374
  pilT Acinetobacter baumannii D1279779

41.42

90.374

0.374
  pilT Acinetobacter baumannii strain A118

41.42

90.374

0.374
  pilT Pseudomonas aeruginosa PAK

41.42

90.374

0.374
  pilT Acinetobacter baylyi ADP1

40.882

90.909

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.828

90.374

0.369
  pilT Legionella pneumophila strain Lp02

40.828

90.374

0.369
  pilT Legionella pneumophila strain ERS1305867

40.828

90.374

0.369