Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   U0039_RS18650 Genome accession   NZ_CP139964
Coordinates   3943831..3944238 (-) Length   135 a.a.
NCBI ID   WP_024956772.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain HAMBI_2659     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3938831..3949238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0039_RS18640 (U0039_18635) pilR 3940375..3941748 (+) 1374 WP_005410795.1 sigma-54 dependent transcriptional regulator Regulator
  U0039_RS18645 (U0039_18640) pilB 3941874..3943607 (-) 1734 WP_024956773.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U0039_RS18650 (U0039_18645) pilA/pilAI 3943831..3944238 (-) 408 WP_024956772.1 pilin Machinery gene
  U0039_RS18655 (U0039_18650) - 3944391..3944828 (-) 438 WP_024956771.1 pilin -
  U0039_RS18660 (U0039_18655) pilC 3945181..3946440 (+) 1260 WP_024956770.1 type II secretion system F family protein Machinery gene
  U0039_RS18665 (U0039_18660) - 3946448..3947311 (+) 864 WP_012481100.1 A24 family peptidase -
  U0039_RS18670 (U0039_18665) coaE 3947323..3947934 (+) 612 WP_024956769.1 dephospho-CoA kinase -
  U0039_RS18675 (U0039_18670) - 3948194..3948580 (-) 387 WP_024956768.1 hypothetical protein -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 14252.43 Da        Isoelectric Point: 6.2109

>NTDB_id=912034 U0039_RS18650 WP_024956772.1 3943831..3944238(-) (pilA/pilAI) [Stenotrophomonas maltophilia strain HAMBI_2659]
MNTQKGFTLIELMIVVAIIAILAAIAAVAFQPYVVRTQLAAALADITPGKTKVEILVNENRPASLITPEFIGGAQNAHCS
NVEAELSDSGVGHISCTLKGHSALDGKDLILRRSAEGIWSCDGSAFEARYRPTGC

Nucleotide


Download         Length: 408 bp        

>NTDB_id=912034 U0039_RS18650 WP_024956772.1 3943831..3944238(-) (pilA/pilAI) [Stenotrophomonas maltophilia strain HAMBI_2659]
ATGAACACGCAGAAGGGCTTCACGCTCATCGAACTGATGATCGTGGTGGCCATCATCGCCATCCTTGCGGCGATTGCGGC
TGTGGCATTTCAGCCATACGTCGTGCGCACCCAGTTGGCGGCGGCACTTGCGGATATCACGCCGGGAAAGACCAAGGTGG
AAATCCTCGTCAACGAGAACCGTCCGGCCAGTCTGATCACCCCCGAGTTCATCGGTGGCGCCCAGAACGCCCACTGCTCC
AACGTCGAAGCCGAACTCAGCGATTCCGGCGTCGGCCATATCAGCTGCACCTTGAAGGGACACAGCGCGCTGGACGGCAA
GGACCTGATCCTGCGCCGCTCCGCCGAGGGCATCTGGAGCTGCGATGGCAGCGCGTTCGAAGCGCGCTATCGCCCGACCG
GCTGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

50.735

100

0.511
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.382

100

0.437
  pilA Pseudomonas aeruginosa PAK

38.255

100

0.422
  pilA Acinetobacter baumannii strain A118

40.876

100

0.415
  comP Acinetobacter baylyi ADP1

35.57

100

0.393
  pilA Vibrio cholerae C6706

34.694

100

0.378
  pilA Vibrio cholerae strain A1552

34.694

100

0.378
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

34.694

100

0.378
  pilA Ralstonia pseudosolanacearum GMI1000

36.842

98.519

0.363
  pilA2 Legionella pneumophila strain ERS1305867

35.507

100

0.363