Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   U0039_RS18645 Genome accession   NZ_CP139964
Coordinates   3941874..3943607 (-) Length   577 a.a.
NCBI ID   WP_024956773.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain HAMBI_2659     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3936874..3948607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0039_RS18630 (U0039_18625) sucC 3937174..3938343 (-) 1170 WP_024956775.1 ADP-forming succinate--CoA ligase subunit beta -
  U0039_RS18635 (U0039_18630) - 3938701..3940314 (+) 1614 WP_024956774.1 ATP-binding protein -
  U0039_RS18640 (U0039_18635) pilR 3940375..3941748 (+) 1374 WP_005410795.1 sigma-54 dependent transcriptional regulator Regulator
  U0039_RS18645 (U0039_18640) pilB 3941874..3943607 (-) 1734 WP_024956773.1 type IV-A pilus assembly ATPase PilB Machinery gene
  U0039_RS18650 (U0039_18645) pilA/pilAI 3943831..3944238 (-) 408 WP_024956772.1 pilin Machinery gene
  U0039_RS18655 (U0039_18650) - 3944391..3944828 (-) 438 WP_024956771.1 pilin -
  U0039_RS18660 (U0039_18655) pilC 3945181..3946440 (+) 1260 WP_024956770.1 type II secretion system F family protein Machinery gene
  U0039_RS18665 (U0039_18660) - 3946448..3947311 (+) 864 WP_012481100.1 A24 family peptidase -
  U0039_RS18670 (U0039_18665) coaE 3947323..3947934 (+) 612 WP_024956769.1 dephospho-CoA kinase -
  U0039_RS18675 (U0039_18670) - 3948194..3948580 (-) 387 WP_024956768.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62287.23 Da        Isoelectric Point: 5.8722

>NTDB_id=912033 U0039_RS18645 WP_024956773.1 3941874..3943607(-) (pilB) [Stenotrophomonas maltophilia strain HAMBI_2659]
MNAVTTANLVGITGLARRLVQDGALDEATARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDALGDALGGD
DDGMGNLDVAAGDEDLGATGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPSSLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=912033 U0039_RS18645 WP_024956773.1 3941874..3943607(-) (pilB) [Stenotrophomonas maltophilia strain HAMBI_2659]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGGCCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCCGCCGCGCGCCAGCCACTGCCGACCTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACTTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCATACCAACCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTGGAACAGTGGCACGCCAGCCATGACGCCCTTGGCGACGCCCTGGGTGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTGGGCGCCACCGGCGGCGATTCCGGCATCGACGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCCTCGGACATCC
ACTTCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTGGCGCGCGCGCCGGTG
AAGCTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCAACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACAGTGTCGCTGTA
TACCGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACTGCCGAAGATCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACGTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCCTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGCCGCCTGTGCGGCAACTGCAAGCGTCCGTCCAGCCTGCCCGAA
CATGCGCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGACGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCGGCATTGAAGAAG
GCGGCGGCGGGCATCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.828

100

0.551
  pilB Acinetobacter baumannii D1279779

55.536

98.614

0.548
  pilB Legionella pneumophila strain ERS1305867

51.93

98.787

0.513
  pilF Neisseria gonorrhoeae MS11

48.596

98.787

0.48
  pilB Vibrio cholerae strain A1552

48.677

98.267

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.133

0.452
  pilB Vibrio campbellii strain DS40M4

45.343

98.614

0.447