Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   U0448_RS04355 Genome accession   NZ_CP139860
Coordinates   846530..847471 (-) Length   313 a.a.
NCBI ID   WP_000249573.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 21011     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 841530..852471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0448_RS04315 comYH 842016..842969 (-) 954 WP_000345100.1 class I SAM-dependent methyltransferase Machinery gene
  U0448_RS04320 - 843030..843624 (-) 595 Protein_822 class I SAM-dependent methyltransferase -
  U0448_RS04325 comGG/cglG 843761..844174 (-) 414 WP_000265631.1 competence type IV pilus minor pilin ComGG Machinery gene
  U0448_RS04330 comGF/cglF 844152..844613 (-) 462 WP_000250531.1 competence type IV pilus minor pilin ComGF Machinery gene
  U0448_RS04335 comGE/cglE 844576..844878 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  U0448_RS04340 comGD/cglD 844841..845245 (-) 405 WP_000588024.1 competence type IV pilus minor pilin ComGD Machinery gene
  U0448_RS04345 comGC/cglC 845238..845564 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  U0448_RS04350 comGB/cglB 845566..846582 (-) 1017 WP_081570974.1 competence type IV pilus assembly protein ComGB Machinery gene
  U0448_RS04355 comGA/cglA/cilD 846530..847471 (-) 942 WP_000249573.1 competence type IV pilus ATPase ComGA Machinery gene
  U0448_RS04360 - 847547..847912 (-) 366 WP_000286415.1 DUF1033 family protein -
  U0448_RS04365 - 848063..849121 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  U0448_RS04370 nagA 849284..850435 (-) 1152 WP_081570975.1 N-acetylglucosamine-6-phosphate deacetylase -
  U0448_RS04375 - 850588..852405 (-) 1818 WP_001220844.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35564.49 Da        Isoelectric Point: 6.0083

>NTDB_id=911338 U0448_RS04355 WP_000249573.1 846530..847471(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 21011]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=911338 U0448_RS04355 WP_000249573.1 846530..847471(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 21011]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361