Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   U0448_RS04330 Genome accession   NZ_CP139860
Coordinates   844152..844613 (-) Length   153 a.a.
NCBI ID   WP_000250531.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 21011     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 839152..849613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0448_RS04295 - 839456..840280 (-) 825 WP_219336202.1 membrane protein insertase YidC -
  U0448_RS04300 rnpA 840255..840626 (-) 372 WP_000739246.1 ribonuclease P protein component -
  U0448_RS04305 - 840643..840774 (-) 132 WP_000768904.1 hypothetical protein -
  U0448_RS04310 - 840775..841965 (-) 1191 WP_000167766.1 acetate kinase -
  U0448_RS04315 comYH 842016..842969 (-) 954 WP_000345100.1 class I SAM-dependent methyltransferase Machinery gene
  U0448_RS04320 - 843030..843624 (-) 595 Protein_822 class I SAM-dependent methyltransferase -
  U0448_RS04325 comGG/cglG 843761..844174 (-) 414 WP_000265631.1 competence type IV pilus minor pilin ComGG Machinery gene
  U0448_RS04330 comGF/cglF 844152..844613 (-) 462 WP_000250531.1 competence type IV pilus minor pilin ComGF Machinery gene
  U0448_RS04335 comGE/cglE 844576..844878 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  U0448_RS04340 comGD/cglD 844841..845245 (-) 405 WP_000588024.1 competence type IV pilus minor pilin ComGD Machinery gene
  U0448_RS04345 comGC/cglC 845238..845564 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  U0448_RS04350 comGB/cglB 845566..846582 (-) 1017 WP_081570974.1 competence type IV pilus assembly protein ComGB Machinery gene
  U0448_RS04355 comGA/cglA/cilD 846530..847471 (-) 942 WP_000249573.1 competence type IV pilus ATPase ComGA Machinery gene
  U0448_RS04360 - 847547..847912 (-) 366 WP_000286415.1 DUF1033 family protein -
  U0448_RS04365 - 848063..849121 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17849.37 Da        Isoelectric Point: 6.4832

>NTDB_id=911333 U0448_RS04330 WP_000250531.1 844152..844613(-) (comGF/cglF) [Streptococcus pneumoniae strain 21011]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=911333 U0448_RS04330 WP_000250531.1 844152..844613(-) (comGF/cglF) [Streptococcus pneumoniae strain 21011]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

95.425

100

0.954
  comGF/cglF Streptococcus pneumoniae D39

95.425

100

0.954
  comGF/cglF Streptococcus pneumoniae R6

95.425

100

0.954
  comGF/cglF Streptococcus pneumoniae TIGR4

95.425

100

0.954
  comGF/cglF Streptococcus mitis SK321

92.81

100

0.928
  comGF/cglF Streptococcus mitis NCTC 12261

91.503

100

0.915